针刺不同经穴干预心肌缺血模型大鼠下丘脑内单胺类递质的相对特异性  

Relatively Specific Effect of Electroacupuncture of Different Acupoints on Hypothalamic Monoamine Neurotransmitters in Myocardial Ischemia Rats

作　　者：汪克明[1] 刘婧[2] 吴子建[1] 王月兰[1] 陈业农[1] 何璐[1] 蔡荣林[1] 

机构地区：[1]安徽中医学院经脉脏腑相关研究中心,合肥230038 [2]上海中医药大学,上海201203

出　　处：《针刺研究》2011年第3期205-208,F0003,共5页Acupuncture Research

基　　金：安徽省自然科学基金(090413112);国家自然科学基金青年科学基金(30801484)

摘　　要：目的:探讨针刺经穴作用的相对特异性,为经脉脏腑相关与脑的联系提供实验支持。方法:随机从80只SD大鼠中选择10只作为正常组,其余大鼠结扎冠状动脉旋前降支复制心肌缺血动物模型。选取模型复制成功大鼠随机分为模型组、神门组、内关组、太渊组,每组10只。按组分别电针大鼠左侧手少阴心经"神门"穴、手厥阴心包经"内关"穴和手太阴肺经"太渊"穴,每次刺激10 min,连续3 d。采用酶联免疫吸附法检测大鼠下丘脑室旁核区内单胺类递质的含量。结果:模型组大鼠下丘脑室旁核区去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量均明显下降(P<0.01)。电针后,与模型组比较,内关组、神门组NE、DA、5-HT含量均明显升高(P<0.01);太渊组NE含量升高(P<0.05),DA、5-HT含量与模型组比较差异无统计学意义(均P>0.05)。结论:电针"神门"穴、"内关"穴皆可促进心肌缺血大鼠下丘脑室旁核区单胺类递质含量恢复,且二者的效果明显优于电针"太渊"穴,说明不同的经穴之间具有相对特异性的中枢调控物质基础。Objective To observe the effect of electroacupuncture （EA） of ＂Shenmen＂ （HT 7）, ＂Neiguan＂ （PC 6）, etc. on monoamine neurotransmitter contents of hypothalamic paraventricular nucleus （PVN） region in myocardial ischemia （MI） rats, so as to analyze the substantial basis of efficacy specificity of acupoints in improving MI. Methods SD rats were randomized into normal control, model, EA-Shenmen （HT 7）, EA-Neiguan （PC 6） and EA-Taiyuan （LU 9） groups （n = 10/group）. MI model was duplicated by occlusion of the anterior descending branch of the left coronary artery. EA （2 Hz, 1. 1 mA） was applied to left ＂Shenmen＇（HT 7） ,＂Neiguan＂（PC 6） and ＂Taiyuan＂（LU 9） for 10 rain, once daily for 3 days. The hypothalamic PVN region tissue was collected for assaying the contents of norepinephrine （NE）, dopamine （DA） and serotonin （5-HT） by using enzyme linked immunosorbent assay. Results In comparison with the normal control group, the contents of NE, DA and 5-HT in hypothalamic PVN in the model group were decreased significantly （P〈0.01）. Compared with the model group,the contents of NE, DA and 5-HT in hypothalamic PVN in the EA-Neiguan （PC 6） and EA-Shenmen （HT 7） groups, and that of NE in the EA-Taiyuan （LUg） group were increased significantly （P〈0.01, P〈0.05）. The effects of EA-Neiguan （PC 6） and EA-Shenmen （HT 7） were significantly superior to that of EA-Taiyuan （LU 9）in increasing the contents of hypothalamic NE, DA and 5-HT （Pal0.05, P〈0.01）. Conclusion Electroacupuncture of PC 6 and HT 7 can suppress MI-induced decrease of NE, DA and 5-HT contents in the hypothalamic PVN region in rats, which may contribute to its effect in resisting myocardial ischemia. The effects of EA-PC 6 and EA-HT 7 are superior to those of EA-LU 9.

关 键 词：电针 下丘脑室旁核 单胺类神经递质 经穴相对特异性 

分 类 号：R245.97[医药卫生—针灸推拿学]