内源性硫化氢对柯萨奇病毒B_3性心肌炎小鼠心肌内病毒复制的影响  

Effect of endogenous hydrogen sulfide on viral replication of myocardium in coxsackievirus B_3 induced viral myocarditis of mice

作　　者：王敏[1] 阳冠明[1] 

机构地区：[1]广西医科大学第一附属医院儿科,南宁530021

出　　处：《中国临床新医学》2011年第6期514-518,共5页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

摘　　要：目的观察内源性硫化氢(H_2S)对柯萨奇病毒B_3(CVB_3)所致病毒性心肌炎(VMC)小鼠心肌内病毒复制的影响,探讨内源性H_2S在VMC发病机理中的作用。方法雄性Balb/c小鼠通过腹腔接种CVB_3复制VMC模型。75只雄性Balb/c小鼠随机分为5组(每组15只):正常对照组、VMC组及胱硫醚-γ-裂解酶(CSE)不可逆抑制剂-炔丙基甘氨酸(PAG)低、中、高剂量干预组(PAG给药采用皮下注射,PAG低、中、高剂量干预组的PAG剂量分别为:20.0、40.0、80.0 mg·kg^(-1),1次/d,共6次。PAG的首次给药为腹腔接种CVB_3的同时皮下注射PAG)。于腹腔接种CVB_3后第7天处死小鼠。用光镜观察心肌组织的病理变化;用分光光度法测定心肌组织的H_2S含量;用酶免疫吸附法测定心肌组织的CSE活性及血清的心肌肌钙蛋白Ⅰ(cTn Ⅰ)含量;用逆转录-聚合酶链反应方法检测心肌组织CVB_3mRNA表达。结果 VMC组心肌组织的CSE活性、H_2S含量明显低于正常对照组(P<0.01),心肌组织的病理积分、CVB_3mRNA表达水平及血清的cTn Ⅰ含量明显高于正常对照组(P<0.01);PAG低、中、高剂量3个干预组心肌组织的CSE活性、H_2S含量明显低于VMC组(P<0.01),心肌组织的病理积分、CVB_3mRNA表达水平及血清的cTn Ⅰ含量明显高于VMC组(P<0.01)。结论心肌内源性H_2S能抑制CVB_3在心肌内复制。Objective To observe the endogenous hydrogen sulfide （H2S） on viral replication of myocardium in eoxsaekievirus B3 （CVB3 ） induced viral myocarditis （VMC）of mice, and to explore the endogenous H2 S on pathogenesis of VMC. Methods Male Balb / e mice were inoculated intraperitoneally with CVB3 to reconstrute an animal model of myocarditis. Seventy-five male Balb / c mice were randomly divided into five groups （ n = 15 ） ： normal control group, VMC group, D, L-prepargylycine [ PAG, an irreversible inhibitor of cystathionine-γ-lyase （CSE） ] low- dose, middle-dose and high-dose intervention groups, PAG was administered by subcutaneous injection （sc）. The mice were inoculated intraperitoneally with CVB3 while se PAG（the dosage of PAG in three intervention groups were 20. 0,40. 0,80. 0mg · kg^-1, respeetivelly）each day one time for six times. All mice were saerificed at seven days after inoculated intraperitoneally with CVB3. The pathological changes of myocardial tissues were observed with light micro- scope. The content of H2S was determined by spectrophotometry in myocardial tissue. The activity of CSE and content of cardiac troponin [ （cTn I ） were determined by enzyme-linked immunosorhnent assay method in myocardial tissue and serum, respectively. The expression of CVB3 mRNA was detected by reverse transcription-ploymerase chaine reac- tion in myocardial tissue. Results VMC group compared with normal control group：The activity of CSE and content of H2 S in myocardial tissues were significantly lower than those in normal control group （P 〈 0. 01 ）, the pathological scores and expressive level of CVB3 mRNA in myocardial tissues, the content of cTn I in serum were significantly high-er than those in normal control group（P 〈 0. 01 ）. PAG three intervention groups compared with VMC group ：The activity of CSE and content of H2S in myocardial tissues were significantly lower than those in VMC group（ P 〈 0. 01 ）, the pathological scores and expressive level of CVB3m

关 键 词：病毒性心肌炎 柯萨奇病毒B3 胱硫醚-Γ-裂解酶 硫化氢 病毒核酸 炔丙基甘氨酸 

分 类 号：R542.21[医药卫生—心血管疾病]