离心力和离心时间对尿沉渣中红细胞和白细胞成分的影响  

The influence of centrifugal force and time on erythrocytes and leukocytes in urinary sediments

作　　者：彭玉莲[1] 张成[1] 冯妙芙[1] 周厚全[1] 卢燕君[1] 马艳[1] 黄宪章[1] 

机构地区：[1]广东省中医院检验医学部,广州510120

出　　处：《国际检验医学杂志》2011年第8期869-870,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨晨尿和随机尿标本经两种离心方法处理后红细胞和白细胞定量检测结果的可比性。方法以红细胞尿液和白细胞尿液为标本,对随机尿和晨尿分别进行1 500 r/min离心5 min和3 000 r/min离心1 min后,使用显微镜镜检法对沉渣中红细胞和白细胞作定量检测。结果中、低浓度红细胞和白细胞的随机尿沉渣中红细胞和白细胞的定量检测结果差异无统计学意义(P>0.05);高浓度红细胞和白细胞随机尿1 500 r/min离心5 min后沉渣中红细胞和白细胞的定量检测结果明显低于其3 000 r/min离心1 min后的检测结果(P<0.01)。高、中、低浓度红细胞和白细胞晨尿1 500 r/min离心5 min后沉渣中红细胞和白细胞的定量检测结果均高于其3 000 r/min离心1 min后的检测结果(P<0.01)。结论对于晨尿中红细胞和白细胞的检测可采用第3版《全国临床检验操作规程》所规定的方法处理后定量检测尿沉渣;而对于随机尿的检测可采用3 000 r/min离心1min后定量检测尿沉渣。Objective To investigate the comparability of the detecting results of erythrocytes and leukocytes in urine sanguinis and random urinary specimens treated with two different centrifugal methods. Methods Microscopic examination was performed to quantitatively detect the erythrocytes and leukocytes in urinary sediments of random urinary specimens and urine sanguinis,centrifugated at 1 500 r/min for 5 minutes and 3 000 r/min for 1 minute. Results There was no statistical difference of erythroeytes and leukocytes in the urinary sediments of random urinary specimens with moderate or low concentration of erythrocytes and leukocytes （P〉0.05）. The detecting results of erythroeytes and leukocytes in urinary sediments of random urinary specimens with high concentration of erythrocytes and leukocytes, centrifugated at 1 500 r/min for 5 minutes, were significant lower than those of random u rinary specimens,centrifugated at 3 000 r/min for 1 minute （P〈0.01）. The detecting results of erythrocytes and leukocytes in urinary sediments of urine sanguinis with low, moderate or high concentration of erythrocytes and leukocytes, centrifugated at 1 500 r/min for 5 minutes,were significant higher than those of urine sanguinis, centrifugated at 3 000 r/min for 1 minute （P〈0.01）. Conclusion Urina sanguinis could be treated according to the methods defined by National Guide to Clinical Laboratory Procedure, but random urinary specimens should be centrifugated at 3 000 r/min for 1 minute,for the quantitative detection of erythrocytes and leukocytes in urinary sediments.

关 键 词：红细胞 白细胞 尿沉渣 定量 离心力 离心时间 

分 类 号：R446.1[医药卫生—诊断学]