余甘子叶提取物总黄酮的含量测定  

Determination of Total Flavonoids in the Extracts from Leaves of Phyllanthus Emblica L.

作　　者：李舒[1] 钟振国[1] 赖进科[1] 

机构地区：[1]广西中医学院,广西南宁530001

出　　处：《辽宁中医药大学学报》2011年第7期109-110,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广西科学技术厅项目(桂科基0639051桂科攻0718002-2-1)

摘　　要：目的:建立余甘子叶提取物中总黄酮含量的测定方法。方法:以槲皮素为对照品,利用紫外可见分光光度法通过测定吸光度计算余甘子叶提取物中总黄酮的含量。结果:槲皮素在2.375～38μg/mL范围内呈良好的线性关系,回归方程为:A=0.0303C-0.0182,r=0.9999,测得余甘子叶提取物中总黄酮平均含量为51.60%。结论:紫外可见分光光度法简单、快捷、准确、灵敏,可作为测定余甘子叶提取物中总黄酮含量的方法。Objective ： Established methods to measure the extract of total flavonoids in leaves of Phyllanthus emblica L. Methods ： Use Quercetin as the object of reference, applying the ultraviolet-visible spectrophotometry to calculate the extract of total flavonoids in leaves of Phyllanthus emblica L. by measuring the absorbanee. Results ： Quercetin has a good linear relationship in the range of 2.375-38 μ g/mL, the regression equation is ： A= 0.0303C-0.0182, r = 0.9999, the average content of total flavonoids in the extract of leaves of Phyllanthus emblica L. is 51.60%. Conclusions ： The spectrophotometry is simple, fast, accurate, quickly and can be used as a method to determine total flavonoids in the extracts from Leaves of Phyllanthus emblica L.

关 键 词：余甘子叶 总黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]