葡萄糖和果糖的速差动力学光度法同时测定  

Kinetic spectrophotometric method for the simultaneous determination of glucose and fructose

作　　者：詹汉英[1] 许小青[1] 

机构地区：[1]陕西师范大学化学系,陕西西安710062

出　　处：《陕西师大学报（自然科学版）》1999年第3期81-84,共4页Journal of Shaanxi Normal University(Natural Science Edition)

摘　　要：利用葡萄糖和果糖与 ２，４二硝基酚在强碱性介质中反应生成红色化合物的反应速度差，建立了光度法同时测定葡萄糖和果糖的速差动力学法．当 Ｎａ Ｏ Ｈ 浓度为 １６×１０－ ３ ｍ ｏｌ／ Ｌ，２，４二硝基酚浓度为 １２×１０－ ３ ｍ ｏｌ／ Ｌ，分别在 ５０℃下反应 ８ｍ ｉｎ 和 ５５℃下反应 ６ ｍ ｉｎ 时，同时测定葡萄糖的线性范围为 ０～９６０×１０－ ３ ｍ ｏｌ／ Ｌ，果糖的线性范围为０～２００×１０－ ３ ｍ ｏｌ／ Ｌ．对葡萄糖与果糖浓度比为１５∶１～１∶１ 的混合液进行回收试验，回收率为９６３％ ～１０５３％ ．A kinetic spectrophotometric method for the simultaneous determination of glucose and fructose is developed.this method based on the rate difference of reactions both with 2,4 dinitropenol in strongly alkaline and measuring the absorbency of product at 500.0 nm against regent blank.As reactions run 8 min at 50℃ and 6 min at 55℃,linear calibration graphs are obtained in the ranges 0~9.60×10 -3 mol/L for glucose and 0~2.00×10 -3 mol/L for fructose. The method had been checked on analysis of binary mixtures (the ratio of concentration glucose/fructose from 15∶1 to 1∶1)with recovery text and successfully applied to determination of glucose fructose syrup.

关 键 词：速差动力学分析 光度法 同时测定 葡萄糖 果糖 

分 类 号：Q532.303[生物学—生物化学] O657.3[理学—分析化学]