禽流感病毒表面抗原HA基因转化小麦的初步研究  

Transformation of wheat with a hemagglutinin gene from H5N1 subtype of avian influenza

作　　者：常向博[1,2] 廖玉才[2,3] 李和平[1,2] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070 [2]华中农业大学麦类作物分子生物技术实验室,武汉430070 [3]华中农业大学植物科学技术学院,武汉430070

出　　处：《华中农业大学学报》2011年第3期266-269,共4页Journal of Huazhong Agricultural University

基　　金："863"国家高技术研究发展专项(2007AA100505)和;教育部博士点基金项目(20070504010)

摘　　要：为了建立利用小麦生产禽流感口服疫苗的方法,选择禽流感病毒H5N1亚型表面抗原HA基因,与谷类作物启动子构建小麦表达载体,经基因枪法转入我国小麦栽培品种郑9023幼胚,在含5 mg/L草铵膦愈伤诱导培养基和分化培养基及含4 mg/L草铵膦生根培养基上筛选后,PCR鉴定获得转HA基因植株,Southern杂交分析转基因T1代植株证实,外源HA基因确已整合到小麦基因组中。To develop a method for the production of avian influenza proteins in wheat as an oral vaccine,a gene encoding hemagglutinin （HA） of avian influenza HSN1 was constructed in a wheat expression vector with promoter of cereal crops. The constructed vector DNA was bombarded into immature embryos of an elite wheat cultivar Zheng 9023. Calluses induced on media supplemented with 5 mg/L phosphinothricin （PPT） were screened and transferred to media containing 4 mg/L PPT for rooting and regeneration. The PPT-resistant green plantlets derived from the rooting media were identified by PCR,and the transgenic wheat plants carrying the HA gene were obtained. Southern blot analysis of T1 transgenic plants demonstrated that the HA gene was indeed integrated into the wheat genome with a single copy integration pattern. These results provide materials for further investigating expression of avian influenza HA gene and accumulation of its protein in wheat seeds.

关 键 词：禽流感病毒 基因枪转化 小麦 植物生物反应器 

分 类 号：S512.150.353[农业科学—作物学]