Total Chemical Synthesis, Assembly of Human Torque Teno Virus Genome  

作　　者：Zheng Hou Gengfu Xiao 

机构地区：[1]（State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071,China）

出　　处：《Virologica Sinica》2011年第3期181-189,共9页中国病毒学（英文版）

基　　金：The Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-Z-3)

摘　　要：Torque teno virus(TTV) is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV(SANBAN isolate) genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerase chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.Torque teno virus（TTV） is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV（SANBAN isolate） genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerase chain assembly reaction（PCA）,and the synthesis was completed with splicing by overlap extension（SOEing）.This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.

关 键 词：Torque teno virus（TTV） Synthetic biology Polymerase chain assembly reaction（PCA） Gene splicing by overlap extension（SOEing） 

分 类 号：S858.31[农业科学—临床兽医学] Q781[农业科学—兽医学]