磷脂酶Dδ缺失加剧UV-B诱导的膜伤害  

Suppression of Phospholipase Dδ Enhances the Membrane Damage Induced by UV-B Irradiation

作　　者：李艳[1,2] 田波[1,3] 李唯奇[1] 

机构地区：[1]中国科学院昆明植物研究所,云南昆明650204 [2]中国科学院研究生院,北京100049 [3]中国科学院西双版纳热带植物园,云南昆明650223

出　　处：《植物分类与资源学报》2011年第3期299-305,共7页Plant Diversity

摘　　要：检测了拟南芥野生型(WS)及磷脂酶Dδ缺失突变体在UV-B辐射下的膜脂分子变化,并比较了二者在紫外辐射下的膜脂含量、双键指数及碳链长度的差异。结果发现,紫外辐射导致植株膜脂发生了降解,其中叶绿体膜脂MGDG和DGDG是膜伤害的主要作用靶点,而且突变体中的膜脂降解比野生型剧烈。上述结果说明磷脂酶Dδ的缺失会加剧紫外辐射诱导的膜伤害,导致植株对紫外辐射更加敏感。The changes of molecular species in membrane lipids under UV-B irradiation in WS and PLDδ-knockout plants were profiled with ESI-MS/MS based lipidomics. The content of membrane lipids, double bond index （DBI） and carbon number of the fatty acid were examined in both of them. The results showed that UV-B irradiation induced the degradation of membrane lipids, in which chloroplast membrane lipids such as MGDG and DGDG were the main targets of membrane injury. In addition, the degradation of membrane lipid in PLD＆def was more severe than that in WS plants. The results suggested that suppression of PLDδ enhanced membrane damage induced by UV-B irradiation.

关 键 词：UV-B 磷脂酶D8 膜脂分子 降解 双键指数 

分 类 号：Q947.9[生物学—植物学]