植物种质资源超低温保存概述  

An Introduction to Cryopreservation of Plant Germplasm

作　　者：文彬[1] 

机构地区：[1]中国科学院西双版纳热带植物园,云南勐腊666303

出　　处：《植物分类与资源学报》2011年第3期311-329,共19页Plant Diversity

基　　金：国家自然科学基金(30571526);中国科学院院长基金

摘　　要：简要回顾了植物种质资源超低温保存的历史,说明了超低温保存植物材料的多样性,阐述了超低温耐性的生物学基础及超低温伤害产生的原因和类型,介绍了各种常用超低温保存方法的技术要点,并对生产顽拗性种子的植物种质资源的超低温保存作了专门的论述,分析了生产顽拗性种子的植物种质资源超低温保存的潜力、现状和困难,指出顽拗性种子的超低温保存是植物种质资源超低温保存的重点和难点,而真正实现用超低温保存技术贮藏顽拗性植物种质资源还有很长的路要走。This paper recited the history of plant germplasm cryopreservation, exhibited the diversity of cryopreservated plant materials, analysed biological foundation of plant cryotolerance, causes and types of cryo-injury, described the key techniques of frequently-used cryopreservation methods, and paid special attention to cryopreservation of plant germplasm of species producing recalcitrant seeds. Based on analysis of potential, status and difficul- ties, the author thought that regard to eryopreservation of plant germplasm of recalcitrant-seeded species there are much to do, for it was the keystone and difficult part of plant germplasm cryopreservation.

关 键 词：种质资源 超低温保存 超低温耐性 超低温伤害 顽拗性种子 

分 类 号：S325[农业科学—作物遗传育种]