大鼠血浆中五味子乙素浓度的HPLC测定法  

Study on a HPLC method for determination of γ-Scchisandrin in rats plasma

作　　者：吴春涛[1] 刘铁楠[1] 张志斐[2] 

机构地区：[1]河北联合大学附属医院,河北唐山063000 [2]河北联合大学药学院,河北唐山063000

出　　处：《华北煤炭医学院学报》2011年第3期310-311,共2页Journal of North China Coal Medical College

摘　　要：①目的建立测定大鼠血浆中五味子乙素浓度的HPLC方法。②方法采用乙腈沉淀血浆蛋白。HPLC的条件为：反相C18（4.6mm×150mm,5μm）色谱柱分离样品,以乙腈-水（78：22）为流动相,流速1.0mL/min,检测波长224nm,柱温23℃。③结果所建立方法在（0.50~50.0）μg/mL范围内线性关系良好（r=0.9975）;平均回收率介于92.60%~103.90%,日内、日间相对标准偏差介于8.17%~12.50%。④结论所用方法简便,准确可靠,适用于五味子乙素大鼠血药浓度测定。Objective To establish a HPLC method for determination of γ-Scchisandrin in rats plasma.Methods Proteins in the plasma were precipitated by adding acetonitrile.The conditions of HPLC method determined：Separation of the samples was carried out on a reversed-phase C18 column（4.6mm×150mm,5μm） with a mobile phase of acetonitrile-H2O（78：22,v/v） at a flow rate of 1.0 mL.min-1.UV detection wavelength was set at 224nm and the temperature of column was kept at 23℃.Results The calibration curve was linear from 0.5～ 50.0μg.mL-1 with correlation coefficient of 0.9975.The recovery of assay was between 92.60% to 103.90%.Both intra-and inter-day coefficients of variation were between 8.17% to 12.50%.Conclusion A rapid and reliable method for determination of γ-Scchisandrin in rats plasma was developed.

关 键 词：高效液相色谱 大鼠 血浆 五味子乙素 

分 类 号：R284[医药卫生—中药学]