TGF-β/Smad信号转导通路对紫外线致人工皮肤光损伤中MMP-1、pro-collagen Ⅰ mRNA表达影响的初步研究  

The Effect of TGF-β/Smad Signal Transduction Pathway on the Expression of MMP-1 and Pro-collagen Ⅰ mRNA in the Process of Photoaging

作　　者：罗雯[1] 姚露[1] 顾华[1] 涂颖[1] 何黎[1] 

机构地区：[1]昆明医学院第一附属医院皮肤科云南省皮肤性病临床研究中心,云南昆明650032

出　　处：《皮肤病与性病》2011年第3期125-128,共4页Dermatology and Venereology

基　　金：云南省科技强省计划项目(2007coo11z)

摘　　要：目的探讨人工皮肤光损伤中TGF-β/Smad信号转导通路与基质金属蛋白酶1及Ⅰ型前胶原蛋白的相互作用。方法以紫外线照射人工皮肤后,通过Real-Time RT-PCR法(荧光染料掺入法)检测其中基质金属蛋白酶1、Ⅰ型前胶原蛋白、TGFβRⅠ及TGFβRⅡmRNA的表达量。结果紫外线使人工皮肤中基质金属蛋白酶1(MMP-1)的mRNA表达量明显增加,而Ⅰ型前胶原蛋白mRNA的表达下调(P<0.05),TGFβRⅡmRNA表达显著下调(P<0.01);加入TGF-β1后MMP-1表达显著下调(P<0.01),Ⅰ型前胶原蛋白及TGFβRⅡmRNA表达显著增高(P<0.01);而UV照射后8小时加入TGF-β1,MMP-1表达量较对照组高(P<0.05),Ⅰ型前胶原蛋白及TGFβRⅡmRNA表达量均较对照组低(P<0.05)。结论 UV可以抑制人工皮肤中Ⅰ型前胶原mRNA的表达、上调MMP-1的表达,而这种调控作用可能与TGF-βRⅡ的表达受抑制,TGF-β/Smad信号传导通路被削弱有关。Objective To investigate the effect of TGF - β/Smad signal transduction pathway on the expression of MMP - 1 and pro - collagen Ⅰ mRNA in the process of photoaging. Methods The skin substitutes including epidermis and dennis was irradiated by UV. For quantitative detection of MMP - 1, pro - collagen Ⅰ, TGF -β type Ⅰ and TGF - β type Ⅱ receptors mRNA expression, a fluorescence based real - time RT - PCR was performed. Results The expression levels of MMP - 1 mRNA were higher（ P 〈 0. 05 ） , but the expression of pro-collagen I and TGF- βR Ⅱ mRNA was down- regulated significantly（ P 〈0. 05） after irradiation by UV. When the skin substitutes were treated with TGF - β1 , the expression levels of MMP - 1 mRNA decreased significantly （ P 〈0. 01 ） , but the expression of pro - collagen Ⅰ and TGF - βR Ⅱ mRNA increased significantly （ P 〈 0. 01 ）. When the skin substitutes were irradiated by UV for 8 hours, then treated with TGF - β1 , the expression levels of MMP - 1 mRNA was higher（ P 〈0. 05 ） , but the expression of pro - collagen Ⅰ and TGFβR Ⅰ mRNA was downregulated. Conclusion The data indicated that UV could suppress TGF - β/Smad signal transduction pathway by inhibiting the expression of TGF - βR Ⅱ mRNA and increase the expression MMP - 1 mRNA, which results in decreased expression of pro - collagen Ⅰ mRNA in uv - imesliated skin substitutes.

关 键 词：紫外线 人工皮肤 Ⅰ型前胶原 基质金属蛋白酶-1 TGF-β/Smad信号传导通路 

分 类 号：R751[医药卫生—皮肤病学与性病学]