维生素A和布地奈德对哮喘大鼠外周血T细胞亚群和调节性T细胞的影响  

The effects of vitamin A and budesonide on serum T-lymphocyte subsets and CD4^+CD25^+ regulatory T cells in rat asthma model

作　　者：何金根[1] 潘家华[1] 楼皖玲[2] 刘辉[1] 

机构地区：[1]安徽医科大学附属省立医院儿科,安徽合肥230001 [2]安徽医科大学生理学教研室,安徽合肥230032

出　　处：《蚌埠医学院学报》2011年第5期452-455,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨维生素A(Vit A)和布地奈德对哮喘大鼠外周血T细胞亚群和CD4+CD25+调节性T细胞(CD4+CD25+regulatory Tcell,CD4+CD25+Treg)的影响,为其在哮喘防治中的应用提供实验依据。方法:将42只SD大鼠用卵蛋白建立哮喘模型,随机分成布地奈德治疗组、Vit A治疗组和哮喘对照组,每组各14只,各组干预1周,于第4周和第5周取外周血50μl,流式细胞术检测T细胞亚群及Treg细胞变化。结果:激发后第4周,Vit A组CD3+T细胞、CD4+CD25+Treg细胞显著高于布地奈德组(P<0.05),CD3+CD8+T细胞均低于布地奈德组(P<0.05);布地奈德组CD4+CD25+Treg细胞显著低于哮喘组(P<0.01);激发后第5周,布地奈德组CD4+CD25+Treg细胞低于哮喘组(P<0.05)。结论:雾化吸入Vit A可减轻哮喘大鼠炎症反应,这一作用可能与哮喘大鼠外周血CD3+T细胞和CD4+CD25+Treg细胞升高、CD8+T细胞降低有关。Objective：To investigate the effects of vitamin A and budesonide on peripheral blood T-lymphocyte subsets and CD4＋CD25 ＋Treg cells in rat asthma model,which provide the experimental basis for vitamin A in the prevention and treatment of bronchial asthma.Methods：Forty-two adult female SD rats were divided into three groups,the budesonide group,vitamin A group and asthma group.There were 14 rats in each group.The peripheral blood was conducted to determinate T-lymphocyte subsets and CD4＋CD25 ＋Treg by flow cytometry.Results：After the 4th of stimulation,CD3＋T cell and CD4 ＋CD25＋Treg cells in vitamin A group were significantly higher than those in budesonide group.CD3＋CD8＋T cell in vitamin A group was significantly lower than that in asthma group（P0.05）.CD4 ＋CD25＋Treg cells of budesonide group was significantly lower than that in asthma group（P0.01）.After the 5th of stimulation,CD4＋CD25＋Treg cells in budesonide group was significantly lower than that in asthma group（P0.05）.Conclusions：Inhalation of vitamin A can reduce inflammation in asthmatic rats,which may be associated with the increases of peripheral blood CD3＋T cells and CD4＋CD25＋Treg cells cells and the decrease of CD8＋T cells.

关 键 词：哮喘 维生素A T细胞亚群 CD4+CD25+调节性T细胞 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]