畲族人群感染乙型肝炎病毒基因型及相关调查  

Distribution and significance of hepatitis B virus genotypes in She population

作　　者：蒋卫平[1] 李旭明[1] 赵志钢[1] 胡晓蕾[1] 张祖平[2] 李梅[2] 吴根凤 

机构地区：[1]浙江省丽水市中心医院,浙江丽水323000 [2]浙江省云和县人民医院,浙江云和323600 [3]浙江省景宁畲族自治县人民医院,浙江景宁323500

出　　处：《中国农村卫生事业管理》2011年第6期623-625,共3页Chinese Rural Health Service Administration

基　　金：浙江省科技计划资助项目(2006C30033);丽水市科技计划资助项目(20072021);项目名称:畲族人群乙型肝炎病毒基因分型及其临床意义

摘　　要：目的:了解丽水地区畲族人群乙型肝炎病毒基因型的分布并比较本地区汉族人群感染该病毒基因型分布的差异,并进一步了解畲族人群乙型肝炎病毒感染者不同基因型对肝脏的损害程度以及乙肝血清标志物的表达。方法:用实时荧光PCR结合S基因测序的方法检测研究对象中HBV DNA阳性感染者,判定其基因型;运用全自动生化分析仪检测其肝功能指标ALT及A ST;用酶联免疫吸附试验检测乙肝血清标志物。结果:共检测了1 744份畲族标本,筛查到HBV DNA阳性173例,其中B型基因78例,C型基因92例,B/C混合型3例;B和C型中HBV DNA拷贝数的平均对数值分别为4.596±1.814和5.364±2.189;B和C型中转氨酶升高的分别为13和15例;B和C型中HB eA g阳性的分别为11和26例;平行检测了相当数量的本地区汉族人群作为对照。结论:丽水地区畲族人群感染HBV以B和C型为主,其中C型基因占的比例大于B型,与同一地区的汉族人群相比具有显著差异;就丽水地区畲族人群而言,B型感染者较C型感染者HBV DNA拷贝数低,HB eA g阳性率低,预示B型感染者较C型感染者的病毒活动性弱,肝脏损害轻,预后好。Objective To investigate the distribution of hepatitis B virus（HBV） genotypes in She population compared with that in Han population also in Lishui area,and to further study the liver injuries and HBV serum markers among She populations infected with HBV of different genotypes.Methods The genotypes of HBV DNA positive subjects were examined by real time fluorescence polymerize chain reaction and S gene sequencing,while ALT and AST by automatic biochemical analyzer and HBV serum markers by ELISA.Results 173 HBV DNA positive samples were screened from 1744 She population,and 78 were genotype B,92 genotype C,3 genotype B/C.The mean HBV DNA copy logarithm of subjects infected with HBV genotype B and C were 4.596±1.814 and 5.364±2.189 respectively.13 of those infected with HBV genotype B and 15 of genotype C were detected with enhanced ALT and AST;and 11 of genotype B and 26 of genotype C were HBeAg positive.The same number of Han populations were also examined as contrasts.Conclusions It can be concluded that She population in Lishui area is mainly infected with HBV genotype B and C,and genotype C takes more proportion,which is obviously different from Han population in the same area.For She population in Lishui,the HBV DNA copy and HBeAg positive rate of HBV genotype B are lower than those of HBV genotype C,which indicates that populations infected with HBV genotype B might have less active virus,lighter liver injuries and better prognosis.

关 键 词：肝炎病毒 乙型 基因型 实时荧光聚合酶链反应 畲族 

分 类 号：R512.62[医药卫生—内科学]