利用弓形虫病人血清筛选弓形虫速殖子特异性抗原  

Screening of Specific Antigen of Toxoplasma Gondii Tachyzoite by Using Human Serum Samples with Toxoplasmosis

作　　者：张影[1] 张瑾[1] 杨英超[1] 薄淑英[1] 辛晓芳[1] 王国治[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2011年第6期550-553,共4页Chinese Pharmaceutical Affairs

摘　　要：目的筛选能引起人体免疫反应的弓形虫抗原标记。方法利用实验室保存的9份弓形虫病人血清标准品、6份阴性血清标准品,以及2份弓形虫病人血清国际标准品作为一抗,分别与刚地弓形虫(Toxoplasma gondii)RH株的可溶性速殖子抗原(soluble tachyzoite antigen,STAg)和排泄-分泌抗原(excreted/secreted antigens,ESA)进行免疫印迹反应。结果发现STAg中有10条蛋白带与所用阳性血清均发生免疫反应;ESA中有4条蛋白带能与所有阳性血清发生免疫反应,这些蛋白与阴性血清均不发生免疫反应。其中分子量为30、35、49、50、54kDa的STAg抗原以及分子量为23、24、28kDa的ESA抗原能与所用阳性血清发生较强免疫反应。结论本实验结果将有助于寻找在弓形虫病人体内能够引起免疫反应的弓形虫诊断抗原和免疫保护性抗原,为弓形虫诊断试剂以及疫苗开发奠定初步基础。Objective Screen antigenic markers of T.gondii tachyzoite evoking immune response in human.Methods Immunoblot was conducted respectively for T.gondii soluble tachyzoite antigen（STAg） and excreted/secreted antigens（ESA） against 17 human serum standards,which includes 9 human serum standard for Anti-Toxoplasma IgG and 6 negative serum standards of our laboratory,and 2 international human serum standard for Anti-Toxoplasma IgG.Results We detected 10 bands in STAg and 4 bands in ESA can react with all positive human serum,which were not reactive with negative human serum.The antigenic bands of apparent MW 30,35,49,50,54kDa of STAg and 23,24,28kDa of ESA reacted distinctly with all human serum with toxoplasmosis.Conclusion The results will help us to find diagnose marker and immunoprotection antigen which can cause immune response in human with toxoplasmosis and lay the initial foundation for the development of diagnostic reagent and vaccinum of T.gondii.

关 键 词：刚地弓形虫 可溶性速殖子抗原 排泄-分泌抗原 血清标准品 免疫印迹 

分 类 号：R382.33[医药卫生—医学寄生虫学] R385[医药卫生—基础医学]