大丽轮枝菌拮抗细菌多粘芽孢杆菌7-4菌株的筛选与鉴定  

Screening and Identification of Antagonistic Bacterium Strain 7-4 against Verticillium dahliae

作　　者：王笑颖[1] 孟成生[1] 雷白时[2] 

机构地区：[1]河北农业大学农学院,河北保定071001 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《湖北农业科学》2011年第9期1797-1799,1825,共4页Hubei Agricultural Sciences

基　　金：河北省自然科学基金项目(398152)

摘　　要：为筛选对棉花黄萎病病原菌大丽轮枝菌(Verticillium dahliae)具有拮抗作用的芽孢细菌,采用改良的琼脂平板扩散法,通过初筛从各地的土样中分离到84株拮抗细菌菌株,再对其进行复筛,得到1株具有较强抑菌活性的拮抗细菌7-4菌株,并对该菌株进行了形态特征观察和生理生化鉴定,初步判断其为多粘芽孢杆菌(Bacillus polymyxa)。In order to obtain antagonistic bacteria against Verticillium dahliae,84 antagonistic strains were isolated from the soil in different areas and screened by improved agar plate diffusion method.The antagonistic activity against V.dahliae of strains were tested via secondary screening.As a result,a strain called 7-4 which had strong inhibition capability against V.dahliae was obtained.According to morphological and culture characters,physiological and biochemical experiments,the strain 7-4 was preliminarily identified as Bacillus polymyxa.

关 键 词：大丽轮枝菌 拮抗细菌 筛选 鉴定 多粘芽孢杆菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]