不同运动方式对大鼠异柠檬酸脱氢酶和磷酸果糖激酶-1的影响  

Effect of different training methods on the activity of isocitrate dehydrogenase and phosphofructokinase-1 in rats skeletal muscle

作　　者：王丽艳[1] 张敏[2] 赵晓丽[1] 郭彦青[1] 曹颖[1] 于公元[1] 康英姿[1] 

机构地区：[1]天津医科大学生物化学教研室,天津300070 [2]武警医学院生物化学教研室,天津300162

出　　处：《天津医科大学学报》2011年第2期170-172,共3页Journal of Tianjin Medical University

摘　　要：目的:探讨不同运动形式对大鼠有氧氧化关键酶-异柠檬酸脱氢酶和酵解关键酶-磷酸果糖激酶-1的影响。方法:72只SD雄性大鼠采用Bedford TG标准的跑台运动方式建立对照组(C)、无氧(E1)和有氧(E2)运动3组模型;每组模型的大鼠随机分为3组:运动2周组、运动4周组和运动6周组。采用酶联法测定异柠檬酸脱氢酶(IDH),6-磷酸果糖激酶-1(PFK)的活性。结果:无氧组IDH和PFK的活性随运动时间的延长均逐步增加,有氧组IDH活性增加,PFK活性降低。IDH活性在运动4周组酶活性最高。结论:与有氧组相比,无氧运动更能有效提高糖酵解和有氧氧化代谢能力,可以作为一种有效方式来指导人们训练。Objective： To investigate the effect of different training methods on the activity of isocitrate dehydrogenase and phosphofructokinase-1 activities.Methods：Seventy-two Sprague-Dawley rats were randomly divided into three groups： control group（C）,anaerobic training group（E1） and aerobic training group（E2）.The rats in each group training 2,4 or 6 weeks just as Bedford TG described.Isocitrate dehydrogenase（IDH） and phosphofructokinase-1（PFK） activities were determined.Results：Compared to C,E1 increased activity of IDH and PFK.E2 increased IDH activity,but decreased PFK activity.The activity of IDH in 4 weeks training group was higher than that in the others.Conclusion：Anaerobic training have better performance in enhancing the zymolysis and the oxidative oxidizing ability,so this training methods may serve as guidance for training program.

关 键 词：大鼠 异柠檬酸脱氢酶 磷酸果糖激酶-1 

分 类 号：R455[医药卫生—运动医学]