羧甲基壳聚糖银的制备及对大鼠牙槽骨吸收的影响  

The preparation of silver carboxymethyl chitosan and the effect of its on ABL in rats

作　　者：金文 王少鹏[2] 佟巨慧[3] 张薷文[3] 费瑞[3] 

机构地区：[1]延边精工口腔医院,吉林延吉133000 [2]黑龙江省农业科学院,黑龙江哈尔滨150086 [3]吉林大学医学部,吉林长春130021

出　　处：《东北师大学报（自然科学版）》2011年第2期129-133,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划重点项目(20100920);长春市科技发展计划项目(08QT10)

摘　　要：经碱化、氯乙酸修饰和离子交换制备了羧甲基壳聚糖银（CMCT-Ag＋）,根据吸附前后溶液中金属离子浓度的变化计算吸附量,通过改变反应时间、取代度、温度和pH值测定了羧甲基壳聚糖（CMCT）对Ag＋的吸附影响;另外,通过大鼠牙槽骨吸收模型分别检测了不同浓度的CMCT-Ag＋对大鼠牙槽骨吸收（ABL）的影响.结果表明：CMCT-Ag＋收率为92.4%,取代度为0.98,Ag＋含量为10.21%.CMCT-Ag＋为粉红色粉末,不溶于水和乙醇,但溶于稀酸.络合反应时间对Ag＋吸附量影响不大;而升温有利于吸附;Ag＋吸附量随—COOH取代度的增加而增大;当溶液pH值达到57时,CMCT与Ag＋络合程度趋于最大.表明环境因素影响CMCT-Ag＋的络合,而控制实验条件可促进Ag＋的络合.牙槽骨吸收实验结果证明,大于100.0 mg/kg的CMCT-Ag＋具有较好的修复大鼠牙槽骨吸收的作用,这为进一步的临床应用奠定了实验基础.CMCT was alkalified,modified with chloracetic acid and exchanged ion to obtain CMCT-Ag＋ complexation.According to the concentration changes of metal ions in adsorption,adsorption quantity was calculated,and Ag＋ adsorption effect was determined by the reaction time,the substitution degree,temperature and pH value.In addition,the alveolar bone loss（ABL） in different group rats,were individually measured.These experiment results showed that CMCT-Ag＋ yield ratio was 92.4%,substitution degree was 0.98 and Ag＋ content was 10.21% in the obtained CMCT-Ag＋.CMCT-Ag＋ was pink powder,and it was insoluble in water and alcohol but soluble in dilute acids.Complexation time impacted little in Ag＋ adsorption;while heating helped adsorption;Ag＋ adsorption was proportional to the substitution degree of -COOH.When pH value of solution reached 57,complexation degree between CMCT and Ag＋ tended to the largest.These showed that environmental factor could influence the complexation of CMCT and Ag＋,and control experimental conditions could promote this process.The experiment results of ALB showed that more 100 mg/kg CMCT-Ag＋ could contribute to repair the ABL,and it will establish experimental foundation for clinical application.

关 键 词：羧甲基壳聚糖银 牙槽骨吸收 吸附量 

分 类 号：Q946.3[生物学—植物学]