外源激素和糖类对玉米zSs1表达的影响  

EFFECTS OF EXOGENOUS PHYTOHORMONES AND CARBOHYDRATES ON EXPRESSION OF zSs1 IN MAIZE

作　　者：刘应红[1] 秦嘉岳[2] 黄小珍[2] 胡育峰[1] 黄玉碧[1,2] 

机构地区：[1]四川农业大学玉米研究所,四川雅安625014 [2]四川农业大学农学院,四川雅安625014

出　　处：《核农学报》2011年第3期432-435,505,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家863项目(2008AA10Z123);国家转基因专项(2009ZX08003-022B)

摘　　要：为明确外源激素和糖类对叶片zSs1表达的影响,本文以玉米优良自交系18-599叶片为材料,在分别含有甘露醇、蔗糖、葡萄糖的培养基中处理,然后分别添加ABA、GA,提取总RNA,通过Real-timePCR检测zSs1基因mRNA表达量变化。结果表明:与只含基础盐处理相比,单独用甘露醇和葡萄糖处理玉米叶片,zSs1基因表达量没有明显变化;而单独用蔗糖处理叶片时,zSs1基因表达量增加约2倍;分别添加ABA或GA后,zSs1基因的表达量也均增加约2倍;蔗糖和ABA或GA的增强作用具有累积效应。In order to study the effect of exogenous hormones and sugars on expression of zSs1 gene in maize,the leaves of maize inbred line 18-599 were soaked in culture mediums,contained mannitol,sucrose and glucose respectively,then treated with ABA or GA and the expression levels of zSs1 mRNA were detected by real-time fluorescence quantitative PCR.The results showed that： compared with those cultured in basic salt,the transcripts of zSs1 didn’t change when treated with mannitol or glucose alone,but increased about 2 times when treated with sucrose.After adding ABA and GA respectively,the expression levels of zSs1 gene was increased about 2-fold either in mannitol,glucose or sucrose,and the increase effects of sucrose and ABA seemed to be a additive.

关 键 词：玉米 淀粉合成酶I 表达 激素 糖类 

分 类 号：S513[农业科学—作物学]