离子对色谱法测定细胞内α-酮戊二酸含量  

Determination of Content of α-Ketoglutarate in E.coli by Ion-Pair Chromatography

作　　者：陶国庆[1] 李凤辰[1] 邵菲[1] 付水林[1] 宫衡[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《化学与生物工程》2011年第6期92-94,共3页Chemistry & Bioengineering

摘　　要：采用离子对色谱法测定细胞内α-酮戊二酸含量。色谱柱为Platisil C18柱,流动相为0.5%(NH4)2HPO4-5mmol.L-1四丁基氢氧化铵,H3PO4调pH值为4.0,检测波长为214 nm。结果表明,α-酮戊二酸与其它代谢物的分离效果较好,方法的线性相关系数为0.9996,相对标准偏差为0.38%,平均回收率为99.38%。An ion-pair chromatography method was established for the determination of content of α-ketoglutarate in E.coli BL21.A Platisil C18 column was used with the mobile phase of 0.5%（NH4）2HPO4-5 mmol·L-1 tetrabutylammonium hydroxide（pH value was adjusted by H3PO4 to 4.0） at the detection wavelength of 214 nm.The results showed that α-ketoglutarate was well separated from other metabolics.Its linear correlation coefficient was 0.9996,the relative standard deviation was 0.38%,the average recovery was 99.38%.

关 键 词：大肠杆菌 离子对色谱法 Α-酮戊二酸 

分 类 号：O657.7[理学—分析化学]