环介导等温扩增技术的研究进展  

Research Progress of Application of Loop-Mediated Isothermal Amplification Method

作　　者：马艳平[1] 刘振兴[1] 郝乐[1] 林敏[1] 孟轩[1] 柯浩[1] 

机构地区：[1]广东省农业科学院兽医研究所广东省兽医公共卫生公共实验室,广东广州510640

出　　处：《河北科技师范学院学报》2011年第1期77-80,共4页Journal of Hebei Normal University of Science & Technology

基　　金：广东省科技计划项目(项目编号:2010B020307003)

摘　　要：环介导等温扩增技术(Loop-mediated isothermal amplification method,LAMP)是一门新兴的基因扩增技术,作为一种分子生物学检测技术,具有高特异性、高敏感性、简单、便捷及成本低的特点,已广泛用于临床疾病的诊断、流行性细菌或病毒的定性定量检测、动物胚胎性别鉴定及基因芯片开发等领域。本研究对环介导等温扩增技术的原理、特性及其应用做一综述,从而为其临床应用提供理论依据。Loop-mediated isothermal amplification（LAMP） is an emerging gene amplification technology.As a detection of molecular biology technology,because of its high specificity and high sensitivity,easy,convenient and low cost,it has been widely used in the diagnosis of clinical disease,bacterial or viral encephalitis qualitative and quantitative detection,animal sex determination and gene chips and so on.This article reviews the principles,characteristics and application of LAMP,with an aim to provide the theoretical basis of its clinical application.

关 键 词：环介导等温扩增技术 原理 应用 

分 类 号：R446.1[医药卫生—诊断学]