两种试剂对儿童血清中梅毒反应素检测的比较与结果分析  

Comparative analysis of serum syphilis reagin in children detected by two reagents

作　　者：谢伟[1] 刘岚[1] 景春梅[1] 赵洁[1] 

机构地区：[1]重庆医科大学附属儿童医院临检中心,400014

出　　处：《检验医学与临床》2011年第11期1310-1310,1312,共2页Laboratory Medicine and Clinic

摘　　要：目的梅毒非特异性血清反应素试验,方法简便快速,可用于梅毒筛查和监测梅毒患者进程和疗效。因此,对本院临检中心使用的检测梅毒患儿血清中反应素两种试剂比较,以选择较好的实验方法,给临床提供更可靠的实验室数据。方法对2009年9月至2010年9月门诊544例患儿血清标本进行两种试剂:梅毒快速血浆反应素诊断试剂(RPR)和梅毒甲笨胺红不加热血清试剂(TRUST)诊断试剂同时检测,其结果进行统计分析。结果两种试剂对544例标本定性试验结果相一致:456例均为阴性,88例均为阳性。88例阳性标本半定量试验:两种试剂85例试验结果相一致,3例标本TRUST试剂抗体滴度比RPR试剂高1个稀释度。结论两种试剂检测的检出结果,差异有统计学意义(P<0.01),具有一致性,对阳性标本滴度的检测TRUST试剂较敏感。Objective To compare the result of two serum syphilis regain reagents in order to select a better method and to provide a more reliable laboratory data for clinic.Methods Serum specimens of 544 cases from September 2009 to September 2010 in out-patient of our hospital were tested by two reagents,including rapid plasma reagin syphilis diagnostic reagent（RPR）and syphilis toluene amine reagent red unheated serum（TRUST）,then we analyzed the result.Results The results of quantative tests of 544 samples by the two reagents were consistent,456 cases were negative,and 88 cases were positive.Within 88 semi-quantitative positive cases,the results of 85 cases were consistent.3 specimens were 1 dilution higher in TRUST kit.Conclusion The result of the two reagents is consistent（P〈0.01）,but TRUST is more sensitive for the positive sample.

关 键 词：儿童 梅毒 反应素 梅毒快速血浆反应素诊断试剂 梅毒甲笨胺红不加热血清试剂 

分 类 号：R457.1[医药卫生—治疗学]