奶牛乳腺中瘦素及其受体的表达与定位  

Expression and localization of leptin and its receptor in mammary gland of dairy cow

作　　者：郭洪波[1] 林叶[1] 李庆章[1] 边艳杰[1] 肖阳[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2011年第6期8-11,共4页China Dairy Industry

基　　金：国家自然科学基金资助项目(30671538);东北农业大学创新团队项目(CXT005-1-1/CXT005-1-2)

摘　　要：为探讨瘦素在奶牛乳腺发育、泌乳及退化各时期的表达变化规律及在乳腺组织中的具体位置,采用免疫印迹(western blot-ting)技术及激光共聚焦技术检测奶牛乳腺组织中瘦素及瘦素受体(OB-Rb)的表达变化及其定位。结果表明,瘦素在青春期表达量较高,在泌乳期表达量最低。瘦素受体在青春期和妊娠期表达量较高,泌乳期表达量相对较低,退化期逐渐恢复到妊娠期水平。The protein expression and localization of leptin and its long form receptor（OB-Rb） were detected by western blottig methord and a confocal laser scanning microscope.The results showed that leptin protein level was higher in virgin,the lowest in lactation.Then OB-Rb was higher in virgin and pregnancy,lower in lactation,and recovered to the original level in involution.

关 键 词：奶牛 乳腺组织 瘦素 长型瘦素受体 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]