骨关节炎软骨退变与Krüppel样因子-5和基质金属蛋白酶-9表达的关系  

Elationship of kriippel-like factor 5 and matrix metalloproteinase-9 expression and neurovascular invasion in osteoarthritis

作　　者：李晗[1] 陈百成[1] 苗穗兵[2] 王飞[1] 邵德成[1] 

机构地区：[1]河北医科大学第三医院关节外科,石家庄050051 [2]河北医科大学生物化学与分子生物学研究室神经与血管教育部重点实验室

出　　处：《中华实验外科杂志》2011年第7期1079-1080,共2页Chinese Journal of Experimental Surgery

基　　金：河北省自然科学基金资助项目（C2010000573、C2009001185）

摘　　要：目的观察Krtippel样因子-5（KLF-5）和基质金属蛋白酶-9（MMP-9）表达活性与人膝关节软骨血管及神经纤维侵入之间的关系。方法从20例行全膝关节置换术的患者中，选取切除的31个关节标本；将每个标本切割成2～3块，并分成磨损组（n=29）和无磨损组（n=17）。结果在磨损组的关节软骨潮线附近有血管化发生，扫描电镜也证实血管结构的存在；关节软骨的神经侵袭仅发生于血管化的标本；与无磨损的关节软骨区比较，磨损区的血管化和神经支配数量明显增加（P〈0．05）。在磨损组的关节软骨中，KLF-5、MMP-9的表达活性明显高丁无磨损组（P〈0．05），而且与血管侵袭程度正相关。结论增加的KLF-5和MMP-9表达可能：是导致骨关节炎软骨退变和血管化的重要机制之一。Objective To investigate the relationship between the expression of krtippel-like factor （KLF）-5, matrix metalloproteinase-9 （MMP-9） and neurovascular invasion in osteophytes in osteoarthritis （OA）. Methods Thirty-one articular cartilage samples were collected from 20 patients who had undergone total knee arthroplasty （TKA）, and each sample was divided into two groups： no change （NC ,n = 17 ） and severe change （ SC, n = 29） according to Mankin score and safranin O staining. Neurovascular markers protein gone product （PGP） 9. 5 and CD34, and the expression of KLF-5, MMP-9 were detected by using immunohistochemistry. Results Vascular channels were observed in both NC and SC sections. In SC sections, 16/29 （55.2%） sections displayed vessels entering the calcified cartilage, which was more than that in NC group （2/17, 11.8% , P 〈 0. 05 ）. The frequency of neurovascular invasion was significantly different between SC and NC （ P 〈 0. 05 ）. Innervation always accompanied vascular invasion at the osteoehondral junction. The severity of neurovaseular invasion was positively correlated with the expression of KLF-5 and MMP-9 in ehondrocytes at the same site that was significantly different between SC and NC. Conclusion KLF-5-induced MMP-9 expression may be involved in cartilage matrix degradation and vascu- larization.

关 键 词：骨关节炎 软骨退变 血管化 KLF-5 

分 类 号：R684.3[医药卫生—骨科学]