慢性压迫性脊髓损伤后大鼠运动功能及周围神经和骨骼肌中胰岛素样生长因子-1表达的变化  

Changes in motor function and expression of insulin-like growth factor-1 of skeletal muscle and peripheral nerve of rats after chronic spinal cord compression

作　　者：李利军[1] 宋洁富[1] 魏杰[1] 常峰[1] 苏云星[1] 王清华[2] 

机构地区：[1]山西省人民医院骨科,太原030012 [2]山西省人民医院肾内科,太原030012

出　　处：《中华实验外科杂志》2011年第7期1084-1086,共3页Chinese Journal of Experimental Surgery

基　　金：山西省卫生厅科技攻关项目（200936）

摘　　要：目的观察慢性压迫性脊髓损伤后大鼠运动功能变化及周围神经和骨骼肌中胰岛素样生长因子-1（IGF-1）表达变化。方法将50只Wistar雌性大鼠随机分为正常组、假手术组和慢性压迫组。慢性压迫组置入平头塑料螺钉对大鼠脊髓进行后路渐进性压迫，于2个月后分别压迫至20％、40％、60％左右程度。行神经功能观察；处死大鼠后取腓肠肌作为标本，分别进行IGF-1的免疫组织化学和原位杂交染色。结果大鼠后肢瘫痪程度随压迫程度加重而加重，各组坐骨神经和骨骼肌中IGF-1蛋白和mRNA表达分别为：20％组（236．9±3．2）、（231．5±2．9）、（245．6±3．4）、（246．6±2．7）；40％组（205．3±2．7）、（202．2±3．4）、（209．4±2．6）、（214．6±2．5）；60％组（215．4±3．5）、（219．3±4．1）、（231．9±2．3）、（238．5±2．7）。各压迫组坐骨神经及骨骼肌中IGF-1 mRNA和蛋白表达增加，与正常组比较差异有统计学意义（P〈0．05）。结论脊髓压迫性损伤可引起周围神经及骨骼肌中IGF-1表达增加，提示机体调动保护因素以减轻脊髓损伤并促进其再生。Objective To observe the changes in motor function and expression of insulin-like growth factor-1 （ IGF-1 ） of peripheral nerve and skeletal muscle in rats after chronic spinal cord compression. Methods A total of 50 Wistar rats were randomly divided into normal group （n = 10 ）, sham operation group （group A, n = 10） and chronic compressive group （goup B, n = 30）. The rats in group B were given gradual compression on the posterior spinal cord using blunt plastics screw. Compression degree reached 20% （n = 10） , 40% （n = 10） and 60% （n= 10） respectively after two months. The rats were killed, and gastrocnemius muscle cells were removed. The expression levels of IGF-1 protein and mRNA in peripheral nerve and skeletal muscle were detected by immunohistochemistry and hybridization respectively after chronic compressive spinal cord injury. Results The rat hind limb paralysis was exacerbated with the increase of the compression. In the sciatic nerve and skeletal muscle, the expression levels of IGF-1 protein and mRNA were： （236.9 ±3.2）, （231.5 ±2.9）, （245.6 ±3.4）, （246.6 ±2.7） in 20% group; （205.3±2.7）, （202.2±3.4）, （209.4±2.6）, （214.6 ±2.5） in40% group; （215.4 ±3.5）, （219. 3 ± 4. 1 ） , （ 231.9 ± 2.3 ） , （ 238.5 ± 2. 7 ） in 60% group. The expression levels of IGF-1 protein and mRNA in peripheral nerve and skeletal muscle were significantly up-regulated after compression （ P 〈 0. 05 ）. Conclusion The results indicate that body transfers the protective factor to relieve injury of CNS.

关 键 词：脊髓损伤 胰岛素样生长因子-1 周围神经 骨骼肌 

分 类 号：R651.2[医药卫生—外科学]