Claudin-1和Claudin-2在大鼠急性结肠炎黏膜中的表达  

Expression of Claudin-1 and Claudin-2 in colonic epithelium of rats with acute colitis

作　　者：张峻[1,3] 罗燕[1] 孙权[2] 朱尤庆[1] 

机构地区：[1]武汉大学中南医院消化内科,430071 [2]武汉大学中南医院普外科,430071 [3]武汉大学科学技术发展研究院

出　　处：《中华实验外科杂志》2011年第7期1145-1147,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察紧密连接蛋白Claudin-1和Claudin-2在急性结肠炎大鼠模型结肠黏膜中的表达。方法对16只SD大鼠采用三硝基苯磺酸灌肠建立急性结肠炎模型，各取8只分别于1周和2周后观察结肠组织的病理学变化，另取5只正常大鼠作对照。应用量子点免疫标记技术检测两种蛋白在正常大鼠、结肠炎1、2周大鼠结肠黏膜中的表达及分布，测定蛋白表达的荧光强度。结果与正常组比较，急性结肠炎1周时大鼠的Claudin-1和Claudin-2表达均明显增强（荧光强度分别为66．01±25．14比48．12±13．25，70．35±24．36比40．12±9．56，P均〈0．01）且分布更广，2周时表达水平较1周时回落（P〈0．05），其中Claudin-1下降较为明显，Claudin-2仍维持一个较高水平[分别为54．64±14．26和60．87±14．27，与正常组比较，差异均有统计学意义（P〈0．05，P〈0．01）]。Claudin-1和Claudin-2表达的变化与结肠炎病理学观察的结果基本一致。结论急性结肠炎导致肠黏膜的Claudin-1和Claudin-2表达增强，尤以Claudin-2增加明显，两者的变化与急性结肠炎的病理改变密切相关。Objective To study the expression of Claudin-1 and Claudin-2 in colonic epithelium of trinitrobenzene sulfonie acid （TNBS） -induced acute colitis models in rats. Methods Sixteen TNBS-induced colitis models in rats were established, and pathologic changes of colonic epithelium were analyzed after one or two weeks respectively. Fluorescence of quantum dots-labelled Claudin-1 and Claudin-2 expression was detected in colonic epithelial cells of normal, one-week colitis, and two-week colitis rats. Fluorescence intensity and distribubition of fluorescence signals were recorded and analyzed. Results As compared with that in normal rats （n = 5） , both Claudin-1 and Claudin-2 expression was increased in one-week colitis rats （ relative fluorescence intensity ： 66. 01 ± 25. 14 vs. 48. 12 ±1.3.25, 70. 35 ±24. 36 vs 40. 12 ±9. 56, both P 〈0. 01 ） with wider distribution in crypt. In two-week colitis rats, Claudin-1 and Claudin-2 expression levels were dropped, but Claudin-2 expression was decreased less than Claudin-1, and both remained at a higher level than normal rats （54. 64 ±14. 26 and 60. 87 ±1.4. 27, respectively, P 〈0. 05 and P 〈 0. 01, respectively vs normal）. The changes of expression of Claudin-1 and Claudin-2 were in accordance with the results of pathologic scoring for colitis. Conclusion Along with the pathologic changes in colonic epithelimn, Claudin-1 and Claudin-2 expression levels are increased in acute colitis rats, and Claudin-2 expression seems more significant than Claudin-1 as an indicator for colitis.

关 键 词：CLAUDIN 实验性结肠炎 炎症性肠病 

分 类 号：R285.5[医药卫生—中药学]