大鼠30％小体积肝移植模型手术技巧  

The techniques of 30 % small-for-size liver transplantation in rats

作　　者：汪小辉[1] 沈柏用[1] 王以巧[1] 鲍国清[1] 詹茜[1] 彭承宏[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院肝移植中心肝胆胰外科,200025

出　　处：《中华实验外科杂志》2011年第7期1179-1181,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：上海市科委基金重点资助项目（09411952100）

摘　　要：目的建立稳定、理想的大鼠30％体积肝移植模型。方法二袖套法和体内切肝法建立非动脉化30％体积肝移植模型，对100只SD大鼠施行50例30％体积肝移植（10例前期操作，不列入统计），对手术操作技巧和细节、术后存活及原因等进行探讨。结果手术成功率100％（40／40），术后1周、2周存活率均40％（16／40），死亡原因主要为肝功能：失代偿、胆管梗阻或胆漏、肝上下腔静脉血栓形成、肝叶坏死和腹腔感染等。结论30％体积肝移植模型改进后供肝残面出血率低，机械损伤小，供肝再灌注色红均匀，存活率好，可作为小体积肝移植有关实验研究的动物模型。Objective To establish a reliable and ideal model of 30% size graft liver transplantation in rats. Methods The model of 30% size graft liver transplantation in vivo was established by using male SD rats according to Kamada＇ s two-cuff method. The operative techniques and ditails, and survival rate were explored and analyzed. Results The rate of successful surgeiT was 100% （40/40）. The one- and two-week survival rate both were 40% （16/40） , and the causes of deaths included liver function decompensation, bile duct stenosis or bile leakage, thrombosis of super inferior vena cava, liver necrosis and celiac infection. Conclusion Improved model of 30% small-for-size liver transplantation had a low hemorrhage rate of residual liver surface, reduced mechanical damage in small-for-size graft, high success rate and survival rate, and was an ideal animal model on the study of experiments related to the small-for-size liver transplantation.

关 键 词：肝移植 小体积 模型 动物 大鼠 

分 类 号：R657.3[医药卫生—外科学]