海藻硫酸多糖及其与Fe^(3＋)络合物对氧化应激THP-1细胞的防护作用  

THE PROTECTIVE EFFECTS OF SEAWEED SULFATED POLYSACCHARIDE AND ITS IRON COMPLEX ON OXIDATIVE STRESSED THP-1 CELLS

作　　者：童静[1] 先宏[1] 陈国庆[2] 丛建波[1] 王长振[1] 吴可[1] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所,北京100029 [2]北京放射与辐射医学研究所,北京100850

出　　处：《营养学报》2011年第3期265-269,共5页Acta Nutrimenta Sinica

基　　金：北京市优秀人才培养资助个人项目(No.2009D013001000028);国家高技术研究发展计划(No.2009AA045401)

摘　　要：目的探讨海藻硫酸多糖(sulfated polysaccharide,SP)及其与Fe3+反应生成海藻多糖铁络合物(seaweed sulfated polysaccharide iron complex,SPIC)对氧化应激THP-1细胞的作用。方法用化学检测法比较SP和SPIC主要成分。在此基础上,用分光光度法检测SPIC中铁离子的离解;用电子自旋共振(electron spin resonance,ESR)自旋捕捉技术对络合前后物质的羟自由基清除作用进行对比;用ESR自旋捕捉技术检测氧化应激细胞呼吸爆发的变化;用MTT(methyl thiazolyl tetrazolium,MTT)法观察络合反应前后对正常THP-1细胞和对氧化应激THP-1细胞的存活率的影响。结果 SP与Fe3+反应形成络合物SPIC后,主要作用成分有较大变化,主要是Fe3+浓度含量显著升高,硫酸根和蛋白含量明显下降,而总多糖变化较小。Fe3+离解实验表明4h内SPIC中的铁能全部溶出;ESR自旋捕捉技术表明,络合前后对羟自由基清除作用与浓度密切相关;与SP相比,在相同浓度时SPIC对氧化应激后的细胞存活更高些。结论 SPIC络合上Fe3+后,在一定浓度时对氧化应激细胞的保护作用更佳,主要与络合后的离子性质和浓度密切相关。Objective To investigate the effects of sulfated polysaccharide from seaweed（SP） and its iron（III） complex（SPIC） on oxidative stressed THP-1 cells.Methods The main components in SP and SPIC were comparatively investigated by chemical analysis,based on which the dissolution of Fe（III） ion from SPIC was detected by spectrophotometric method.The hydroxyl radical scavenging effect of SP and SPIC was observed and compared with the help of ESR spin trapping technique in vitro,so did the respiratory burst on the THP-1 cells.The viability of normal cell and oxidative stressed cell was also compared between the two compounds using MTT method.Results The main composition changed greatly after ferric ion was chelated to SP as SPIC,mainly due to that ferric ion was increased markedly while sulfate and protein contents decreased significantly,but total polysaccharide changed slightly.Ferric ions in SPIC could be all dissolved in 4 h.ESR spectra showed that both SP and SPIC had effect on scavenging hydroxyl radicals,closely dose-dependent.In comparison with SP,SPIC could promote the viability of oxidative stressed cells better at the same concentration.Conclusion In comparison to SP,SPIC has effectively protective effects on oxidative stressed cell,which is closely concentration dependent.

关 键 词：海藻硫酸多糖 铁离子 氧化应激 THP-1细胞 

分 类 号：R285.5[医药卫生—中药学]