止喘汤对哮喘大鼠模型气道重构中MMP-9、TIMP-1表达的影响  

Effects of Zhichuan decoction on MMP-9,TIMP-1 during airway remodeling in asthmatic rats

作　　者：霍博雅[1] 张占锋[2] 

机构地区：[1]辽宁医学院附属第三医院中医科,辽宁锦州121001 [2]辽宁医学院,辽宁锦州121001

出　　处：《中国中医基础医学杂志》2011年第6期627-629,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：辽宁省教育厅资助项目(2008420)

摘　　要：目的:观察中药止喘汤对哮喘大鼠气道重构的干预,并探讨其对基质金属蛋白酶-9(MMP-9)及金属蛋白酶抑制剂-1(TIMP-1)表达的影响。方法:50只Sprague-Dawley(SD)大鼠随机分为正常组、哮喘组、布地奈德(BUD)组、黄芩组及止喘汤组5组。采用卵清白蛋白(OVA)致敏加激发法复制大鼠哮喘模型,苏木精-伊红染色测气道形态学参数,免疫组化染色测定气道壁MMP-9、TIMP-1及IV型胶原含量。结果:哮喘组较正常组大鼠气道壁明显增厚,MMP-9、TIMP-1表达增多(P<0.01)。用中药和BUD干预后大鼠气道壁明显变薄,MMP-9、TIMP-1表达减少,与哮喘组比较有统计学意义(P<0.01);其中复方组肺纤维化轻于单味组,各项指标比较有统计学意义(P<0.05)。大鼠气道壁的厚度、IV型胶原含量、MMP-9、TIMP-1及MMP-9/TIMP间有相关关系。结论:中药具有干预哮喘大鼠模型气道重构的作用,这些作用可能是通过调节MMP-9和TIMP-1而实现的,基于中医基本病机证型的复方优于单味中药。Objective：To investigate the effects of Traditional Chinese Medicine（TCM） on airway remodeling and the expression of MM P-9 and TIMP-1 in asthmatic rats. Methods：Fifty Sprague-Dawley rats were randomly divided into five groups equally ： normal control group, asthmatic group,budesonide aerosol group, scutellaria baiealensis group and Zhichuan decoction group. The model of asthma was established by OVA （ovalbumin）sensitizing and challenging; some lung tissues were sliced and stained with HE and morphological indicators of airway were measured by image analysis, the other lung tissues were sliced and stained with Immunohistoehemistry and the expression of MMP-9 ,TIMP-1 and collagen type IV was observed. Results： Compared with normal controls, the thickness of airway wall in asthmatic models was significantly increased,and the expression of MMP-9 and TIMP-1 was also increased significantly （ P 〈 0. 01 ）. After intervention with TCM and budesonide aerosol, compared with asthmatic models, the thickness of airway became thinner significantly, meanwhile,the expression of MMP-9 and TIMP-1 was significantly decreased （P 〈 0.01 ）;then compared the two TCM, pulmonary fibrosis of intervention with compound medical herbs was lighter than intervention with the single （ P 〈 0. 05 ）. Airway wall thickness and collagen type IV were associated wil：h MMP-1 ,TIMP-1 and MMP-9/TIMP-1. Conclusions：The TCM could decrease the deposition of collagen type IV and reduce the airway thickness by regulating MMP-9 and TIMP-1 levels and influencing the balance between MMP-9 and TIMP-I, the compound based on asthmatic basic pathogenesis of TCM is superior to single herbs.

关 键 词：气道重构 黄芩 止喘汤 基质金属蛋白酶-9 基质金属蛋白酶抑制剂-1 

分 类 号：R285.5[医药卫生—中药学]