柱层析法提纯羊毛脂中胆固醇的工艺  

Purification of cholesterol from lanolin by column chromatography

作　　者：胡文娜[1] 苏宝根[1] 苏云[1] 杨亦文[1] 任其龙[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,二次资源国家专业实验室,浙江杭州310027

出　　处：《化工进展》2011年第7期1426-1430,共5页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金重点项目(20936005);国家863计划重点项目(2010AA101503)

摘　　要：采用柱层析法分离羊毛脂中的胆固醇,以胆固醇与杂质羊毛甾醇的分离度为判定依据来筛选吸附剂及优化层析工艺,同时对吸附剂进行了再生考察。实验表明,当以200～300目粗孔硅胶为吸附剂、丙酮/正己烷混合溶剂(体积比4∶96)为流动相、35℃下胆固醇上载量为2.4%(质量分数)时,胆固醇的分离效果较好,所得胆固醇含量可达84.36%,收率为85.67%。另外选用丙酮/正己烷混合溶剂(体积比50∶50)对吸附剂进行再生处理,硅胶经7次使用再生循环,分离效果未降低。Cholesterol was purified from lanolin by column chromatography.The separation process was optimized based on the resolution between cholesterol and lanosterol,which was one of the impurities.The regeneration of adsorbent was also studied.The optimum purification condition was as follows：adsorbent was silica gel,mixture solvent of acetone/hexane（4∶96,volume ratio）was used as eluent and proper temperature was 35℃.After the separation,the purity of cholesterol was determined by HPLC to be 84.36% and the recovery was 85.67% when the column charge of cholesterol was 2.407% by weight.Additionally,the adsorbent was regenerated by the mixture solvent of acetone/ hexane（50∶50,volume ratio）.The separation ability of silica gel remained almost unchanged after 7 cycles of usage and regeneration.

关 键 词：胆固醇 羊毛甾醇 柱层析 硅胶 再生 

分 类 号：TQ645.9[化学工程—精细化工]