溶剂浮选分离-紫外分光光度法测定环境水样中罗红霉素残留量  

UV-Spectrophotometric Determination of Roxithromycin in Environmental Water Samples with Separation by Solvent Floatation

作　　者：戈延茹[1] 曹恒杰[1,2] 傅海珍[1] 张晓兰[1] 闫永胜[3] 

机构地区：[1]江苏大学药学院,镇江212013 [2]江苏济川制药有限公司药物研究所,泰兴225400 [3]江苏大学化学化工学院,镇江212013

出　　处：《理化检验（化学分册）》2011年第6期653-655,658,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用溶剂浮选法分离富集环境水样中罗红霉素,并利用紫外分光光度法对罗红霉素进行测定。罗红霉素的最佳浮选条件为在磷酸氢二钾溶液中,以异丙醇作浮选溶剂,控制溶液pH为9.0,氮气流量15mL·min^(-1),浮选时间30min。罗红霉素的质量浓度在8.2～40.2mg·L^(-1)范围内与其吸光度呈线性关系,检出限(3S/N)为0.26mg·L^(-1)。在河水及湖水样品中分别加入3个浓度水平的罗红霉素标准溶液做方法的回收试验,测得平均回收率均超过90%。Roxithromycin in environmental water was separated and enriched by solvent floatation, and determined by UV spectrophotometry. Floatation was carried out in a K2 HPO4 solution of pH 9.0 with isopropanol as floatation solvent and by passing N2-gas through the solution at the rate of 15 mL . min 1 for 30 min Linear relationship between values of absorbance and mass concentration of roxithromycin were obtained in the range of 8. 2-40. 2 mg . L ^-1, with detection limit （3S/N） of 0. 26 mg . L^-1. In testing for recovery by addition of standard roxithromycin solution at 3 concentration levels to samples of river and lake water, values of average recovery more than 90% were obtained.

关 键 词：紫外分光光度法 溶剂浮选 罗红霉素 环境水样 

分 类 号：O657.32[理学—分析化学]