莪术油眼用凝胶对兔腺病毒角膜炎的药效学研究  

Pharmacodynamics Study of Zedoary Oil Ophthalmic Gel on Rabbit's Adenovirus Keratitis

作　　者：曹高忠[1] 刘敏[2] 陈熙[3] 汤从容[1] 蔡剑秋[4] 梁晶[1] 叶晓兰[1] 

机构地区：[1]温州医学院附属第一医院药剂科,浙江温州325000 [2]温州医学院药学院,浙江温州325000 [3]温州医学院附属眼视光医院整形科,浙江温州325000 [4]温州医学院附属第二医院眼科,浙江温州325000

出　　处：《中国药师》2011年第6期781-783,共3页China Pharmacist

基　　金：温州市科技计划项目(编号:Y20090174)

摘　　要：目的:评价莪术油眼用凝胶对兔腺病毒性3型角膜炎的治疗作用。方法:造模成功后,将实验动物随机分为腺病毒感染未治疗组(0.9%氯化钠注射液滴眼)、阿昔洛韦眼用凝胶治疗组和莪术油眼用凝胶治疗组3组,每组6只(12只眼),另取6只正常非感染病毒白兔作为空白对照组(0.9%氯化钠注射液滴眼)。于实验的第3,第6,第10d在裂隙灯显微镜下观察白兔眼部病变的变化。结果:用药后第3,第6及10d观察,未治疗组(角膜病变分值3.75、3.67及3.58)兔角膜病变程度较严重,而阿昔洛韦眼用凝胶治疗组(2.92、1.83及0.75)和莪术油眼用凝胶治疗组(2.33、1.08及0.25)兔角膜病变较轻(P<0.01或0.05),尤其莪术油眼用凝胶治疗组兔角膜病变程度最轻(P<0.01),且在用药后第10天兔角膜病变康复得最好。用药10d后观察兔角膜上皮松解、脱落,而阿昔洛韦眼用凝胶治疗组和莪术油眼用凝胶治疗组角膜上皮完整、细胞排列整齐。结论:莪术油眼用凝胶对兔腺病毒3型角膜炎有明显的治疗作用,可以显著缩小角膜病变面积,促进角膜上皮细胞的修复,其作用优于阿昔洛韦眼用凝胶。Objective： To observe the effects of zedoary oil ophthalmic gel on experimental adenovirus type 3 （ Ad3 ）. Method： The rabbits infected with adenovirus type 3 （Ad3） , were divided randomly into three groups：the untreated group, acyclovir eye gel group, zedoary oil ophthalmic gel group, with 6 rabbits in each group （ 12 eyes）. Another 6 rabbits （ 12 eyes） were in blank control group. The acyclovir eye gel group was treated with acyclovir eye gel, zedoary oil ophthalmic gel group with zedoary oil ophthalmic gel everyday, and both untreated group and control group with 0. 9% saline. The cornea pathological changes was observed by slit lamp corneal microscope at 3,6,10 day respectively. Result： After the adminstration at 3,6 and 10 d, the pathological changes of cornea were most serious in untreated group （corneal involvement scores were 3.75,3.67 and 3.58 ） , however, there was no serious change in acyclovir eye gel group （2.92,1.83 and 0. 75） and zedoary oil ophthalmic gel group （2. 33,1.08 and 0. 25） （P 〈 0. 01 or 0. 05 respectively）. Furthermore, The zedoary oil ophthalmic gel group （ P 〈 0. 01 ） showed the lightest pathological changes, and the cornea was well recovered at 10 d after treatment. The corneal epidermis of rabbits fell off at 10 d,however, the cornea in acyclovir eye gel group and zedoary oil ophthalmic gel group was intact with uniform cell alignment. Conclusion： Zedoary oil ophthalmic gel can be markedly effective in treatment of experimental rabbit adenovirus keratitis. It can reduce the area of pathological cornea and promote recovery of cornea epithelial cell, and its effects are better than acyclovir.

关 键 词：莪术油眼用凝胶 腺病毒性角膜炎 

分 类 号：R285.5[医药卫生—中药学]