HPLC法测定塞克硝唑片的有关物质  

Determination of Related Substances in Secnidazole Tablets by HPLC

作　　者：张金安[1] 郭均平[1] 沈惠[1] 邓民民[1] 

机构地区：[1]武警湖北省总队医院,武汉430061

出　　处：《中国药师》2011年第6期834-835,共2页China Pharmacist

摘　　要：目的:建立HPLC法测定塞克硝唑片中有关物质的方法。方法:色谱柱:Inertsil ODS-3 C_(18)(150mm×4.6mm,5μm);流动相:乙腈-0.03mol·L^(-1)醋酸铵溶液(11:89);检测波长:330nm;流速:0.8ml·min^(-1);柱温:40℃。结果:塞克硝唑在3.95～316.16mg·L^(-1)范围内与峰面积呈良好的线性关系(r=0.9998);其最小检出限为0.4ng。塞克硝唑峰的理论塔板数大于7000,且与杂质峰的分离度大于1.5。结论:本方法的专属性强,准确性高,适宜于分析塞克硝唑片的有关物质。Objective： To establish an HPLC method for determination of related substances in secnidazol tablets. Method： Inertsil ODS-3 Cl8 column （4. 6 mm ×150 mm 5 μm） was used with the mobile phase of acetonitrile and 0. 03 mol·L^-1 ammonium acetate solution （ 11 ： 89）. The detection wavelength was 330 nm, the flow rate was 0. 8 ml· min^-1 and the column temperature was 40 ℃. Result ： The linear relationship of secnidazole was promising within the range of 3.95-316. 16 mg· L^-1（ r = 0. 999 8 ） with the lowest limit of detection of 0. 4 ng. The theoretical plate number was above 7000 with the resolution of the impurities above 1.5. Conclusion： The method is simple,specific and sensitive. It is suitable for the determination of related substances in secnidazol tablets.

关 键 词：塞克硝唑片 有关物质 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]