米诺环素对糖尿病大鼠视网膜神经细胞凋亡的影响  

Effects of Minocycline on Apoptosis of Retinal Nerve Cells of Rats with Diabetic Retinopathy

作　　者：练海东[1] 任兵[2] 高晓唯[2] 郭继华[3] 解玉军[4] 张改丽[4] 赵旭东[4] 刘莹[4] 

机构地区：[1]石河子大学医学院一附院眼科,新疆石河子832000 [2]解放军474医院全军眼科中心,新疆乌鲁木齐830013 [3]解放军474医院病理科,新疆乌鲁木齐830013 [4]石河子大学医学院,新疆石河子832000

出　　处：《现代生物医学进展》2011年第13期2435-2437,共3页Progress in Modern Biomedicine

摘　　要：目的:观察米诺环素对糖尿病大鼠视网膜神经细胞的凋亡的影响,研究米诺环素对糖尿病视网膜神经保护作用,对米诺环素在糖尿病视网膜疾病中抑制神经细胞凋亡提供理论支持。方法:选择健康成年雄性SD大鼠30只,随机分成正常对照组、糖尿病模型组和米诺环素治疗组,每组10只。腹腔内注射链脲佐菌素(STZ)诱发大鼠糖尿病。米诺环素治疗组给予米诺环素腹腔注射(45mg/kg),共注射10d,模型组和对照组腹腔注射等体积生理盐水,于给药后8周的3组动物处死,冰浴下取其视网膜组织,随后制备视网膜石蜡切片,采用末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记(TUNEL)法进行凋亡细胞原位标记,行视网膜神经细胞凋亡计数,对所有数据进行统计学分析。实验结果拟用均数和标准差(x±s)表示,P<0.05为差异有统计学意义。结果:相同观察时相,与阴性对照组比较,模型对照组大鼠视网膜TUNEL阳性细胞显著增多(P<0.01),在米诺环素组,大鼠视网膜TUNEL阳性细胞数比模型对照组明显减少,差异有统计学意义(P<0.01)。结论:米诺环素能有效降低糖尿病大鼠视网膜神经细胞的凋亡,对糖尿病视网膜神经细胞有保护作用。Objective： To study the effect of the Minocycline （MNC） on expression of apoptosis in retinal nerve cells of rat with diabetic retinopathy （DR）. Methods： A total of 30 male SD rats were randomly divided into negative control group,model control group and MNC treated group. Diabetes model was established by intraperitoneal injection of 60 mg/kg streptozotocin （STZ）. Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling （TUNEL staining） was used to observe neuron apoptosis in retina. All the experimental data were expressed by Mean＋standard deviation. Results： Compared with the negative control group, TUNEL positive cells in retina were increased significantly （P〈0.01） in model control group. However, TUNEL positive cells in retina in MNC treated group were significantly decreased （P〈0.01）. Conclusion： MNC can obviously decrease the expression ofapoptosis in retina,with DR. It is one of path of inhibiting impairment on retinal nerve cells with DR.

关 键 词：糖尿病视网膜病变 米诺环素 凋亡 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]