白介素1受体拮抗剂及转化生长因子β1对兔膝关节骨性关节炎(OA)的治疗研究  

Interleukin-1 Receptor Antagonist and Transforming Growth Factor-β1 for Rabbits with Osteoarthritis

作　　者：樊志强[1] 庞炜[1] 杨连甲[1] 文艳华[1] 王海鹏[1] 张小平[1] 周勇[1] 范清宇[1] 

机构地区：[1]第四军医大学唐都医院全军骨肿瘤研究所,陕西西安710038

出　　处：《现代生物医学进展》2011年第13期2447-2450,2408,共5页Progress in Modern Biomedicine

基　　金：军队医药卫生科技项目(LXH-2006010)

摘　　要：目的:通过关节腔内注射白介素1受体拮抗剂(IL-1Ra)及转化生长因子β1(TGF-β1),观察其对骨性关节炎的治疗作用。方法:16只3-4个月龄新西兰大白兔(雌雄不限)随机分为四组,每组四只,分为正常组,对照组,IL-1Ra组,TGF-β1组,正常组仅打开左膝关节关节腔,剩余三组均给予左膝关节前交叉韧带离断术(anterior cruciate ligament transaction ACLT)。四周后IL-1Ra组给予关节腔内注射IL-1 Ra 5g/ml,TGF-β1组给予关节腔内注射TGF-β1 50mg/ml,每周两次,连续四周,正常组及对照组未采取任何措施。术后八周时采用空气栓塞法处死全部实验动物,取股骨远端长约1cm,固定后切片行细胞形态学观察。结果:大体肉眼观察:对照组股骨远端软骨面色颜色变暗并稍显粗糙,可见少量软骨脱落,其余两组关节软骨大体形态均接近正常。镜下观察:TGF-β1组软骨细胞增生明显,软骨细胞排列较正常软骨细胞排列轻度紊乱。IL-1Ra组软骨细胞增生数量未及TGF-β1组明显,软骨细胞排列也不及TGF-β1组规则。IL-1Ra组及TGF-β1组软骨细胞形态接近正常软骨细胞,基质染色接近正常。两组软骨膜表面光滑,无纤维细胞形成,软骨膜下未见纤维化。对照组软骨细胞数量明显减少且排列紊乱,软骨细胞形态发生改变,可见软骨细胞凋亡,镜下可见软骨细胞核浓缩、裂解,并可见新生软骨形成,突出于正常软骨膜表面,软骨膜表面下方可见血管翳及纤维母细胞形成,出现轻度纤维化。基质染色不均匀且染色较淡。结论:IL-1Ra及TGF-β1关节腔内注射均能有效抑制骨性关节炎关节软骨的退变并增加软骨细胞再生,促进软骨基质合成,延缓骨性关节炎的发病进程。Objective： To observation the treatment of rabbit osteoarthritis by vivo interleukin-1 receptor antagonist （IL-1Ra） and transforming growth factor-β1 （TGF-β1）. Methods： A total of 16 three to fourmonth -old New Zealand white rabbits were divided randomly into four experimental groups. Group one （normal group） received only opened the left articular cavity.Those in another three groups（IL-1Ra group,TGF-β1 group and control group） received anterior cruciate ligament transection （ACLT）.Four weeks later, IL-1Ra group,TGF-β1 group were given vivo interleukin-1 receptor antagonist and transforming growth factor-β1 twice one week and anotheor groups did nonthing.All animals were scarificed eight weeks later after surgery.The samples were stained for tissue slice. Results： In general visual study IL-1Ragroup, TGF-β1 group and control group the articula cartilage close to normal. Control group cartilage is stramineous and can find amotic. Observation by microscope： in IL-1Ragroup, TGF-β1 group and control group cartilage cell proliferation obviously and arrange orderly, the control group cartilage cell decreased and choas and can find fibroplasia.Conclusion： By vivo interleukin-1 receptor antagonist （IL-1 Ra） transforming growth factor-β1 （TGF-β1） can effectively inhibit degeneration of articular cartilage cell increase articular cartilage cell regeneration avilably.This will release clinical symptoms so to delay the process of osteoarthritis.

关 键 词：骨性关节炎 白介素受体拮抗剂 转化生长因子 关节软骨 

分 类 号：R684.3[医药卫生—骨科学]