吸烟大鼠肺脏AQP4和MUC5AC的表达及其与一氧化氮的关系  

Expression of AQP4 and MUC5AC in Lungs of Smoking Rats and Its Relation with Nitric Oxide

作　　者：张亮[1] 孙香蕾[2] 雷蕾[1] 李锋[2] 吴昌归[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710032 [2]第四军医大学西京医院中医科,陕西西安710032

出　　处：《现代生物医学进展》2011年第13期2455-2457,2434,共4页Progress in Modern Biomedicine

基　　金：国家重点基础发展规划项目(973项目)(2009CB522706)

摘　　要：目的:研究烟草烟雾吸入对大鼠肺组织水通道蛋白4(AQP4)和粘蛋白5AC(MUC5AC)表达的影响及其与支气管肺泡灌洗液内一氧化氮代谢物水平的关系,探讨氧化应激对肺部水转运和粘液分泌的影响。方法:免疫组化法观察AQP4在肺组织内的表达,平均光密度法比较模型组和空白组大鼠AQP4的表达差异;半定量RT-PCR法检测肺组织内AQP4及MUC5AC mRNA的表达水平;硝酸还原酶法测定各组大鼠支气管肺泡灌洗液内一氧化氮代谢产物的浓度,分析模型组AQP4、MUC5AC mRNA的表达水平与支气管肺泡灌洗液内一氧化氮代谢物浓度之间的相关关系。结果:AQP4在空白对照组呈强阳性染色,在模型组呈弱阳性染色,两者平均光密度值有显著差异(P<0.05)。模型组动物肺组织AQP4 mRNA的表达降低,MUC5AC mRNA的表达升高,与空白组比较均有显著差异(P<0.05),模型组动物支气管肺泡灌洗液内一氧化氮代谢产物的浓度与肺组织AQP4 mRNA表达水平呈负相关,相关系数r=-0.798(,P<0.05),与MUC5AC mRNA的表达水平呈正相关,相关系数r=0.857(,P<0.05)。结论:吸烟可导致肺组织AQP4表达下降进而影响气道内水的转运。一氧化氮可能参与了烟雾吸入动物模型中AQP4与MUC5AC基因表达的调控。Objective： To investigate the relationship of the expression of AQP4 and MUC5AC in tobacco-smoking and nitric oxide metabolics in BALF, and to investigate the effect of oxidative stress on water transport of lung. Methods： Tobacco-smoking model was established and the expression of AQP4 in lungs was examined by immunohistochemical stain and optical density analysis. AQP4 and MUC5AC mRNA expression was detected by semiquantitive reverse transcription-polymerase chain reaction（RT-PCR）. Nitric oxide （NO） metabolics in BALF was detected by nitrate reductase assay and its relationship with AQP4 and MUC5AC mRNA expression was analyzed by Pearson＇s correlation. Results： There was strong positive expression of AQP4 in control group while it was a weak positive stain in smoking model group. Optical density analysis showed that their difference is statistically significant （P 〈0.01 ）, In smoking model group the expression ofAQP4 mRNA decreased and MUC5AC mRNA increased compared with that in the control group （P〈 0.01 ）. In smoking model group, concentration of NO metabolics from BALF is negatively correlated with AQP4 mRNA level（r=-0.849, P 〈0,01 ） and positively correlated with MUC5AC mRNA level （r=0.857, P 〈0.01 ）Conclusions： Smoking causes the decreasing of AQP4 expression in lung tissue which influences water transport in airways. NO may play a vital role in this process through regulating AQP4and MUC5AC mRNA expression.

关 键 词：水通道蛋白 一氧化氮 粘蛋白5AC 吸烟 

分 类 号：Q95-3[生物学—动物学] R56[医药卫生—呼吸系统]