酪氨酸酶与羊驼毛色相关性的研究  

Relevance between TYR and Different Hair Color of Alpaca

作　　者：高莉[1,2] 赵英虎[2] 刘朝亮[2] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]中北大学化工与环境学院,山西太原030051

出　　处：《激光生物学报》2011年第3期319-323,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目(30501070);中北大学校科学基金项目

摘　　要：为揭示羊驼毛色形成机理以及毛用性状改良奠定基础,选用羊驼作为试验动物群体,以酪氨酸酶作为影响羊驼毛色性状的候选基因,采用荧光定量PCR技术、免疫组织化学、免疫印迹等生物学方法从基因与蛋白方面分析了羊驼酪氨酸酶(tyrosinase,TYR)在不同毛色个体的表达量。荧光定量PCR结果显示TYR基因在棕色个体中的mRNA表达量高于白色个体中。免疫印迹实验与免疫组化实验均表明TYR蛋白在棕色个体中的蛋白表达量高于在白色个体中的蛋白表达量。综上,毛色表型与酪氨酸酶的表达量存在相关性,TYR参与调控了毛色色素沉着过程。The certain biological foundation for the formation mechanism of alpaca hair color and the reforming of color characters were established. Alpaca were used as the experimental animal group. TYR gene was taken as candidate genes affecting the character of alpaca＇ s hair color. TYR expression quantity were analysed by using several biological methods including quantitative real time PCR, immunohlstochemistry and western blot. Results of QRT-PCR showed that, TYR gene expressive quantities in brown alpacas were higher than ones in white alpacas. Results of immunohistochemistry and western blot indicated protein expression quantities of TYR in brown alpaca were higher than ones in white alpaca. To sum up, the phenotype of brown and white color and the expression amount of TYR have relevance, TYR participated in regulating and controlling hair pigmentation.

关 键 词：羊驼 TYR基因 TYR蛋白 

分 类 号：S829.9[农业科学—畜牧学]