枸杞多糖对H5N1流感全病毒灭活疫苗的体液免疫增强作用  

Antibody Responses Induced by Inactivated Whole-virion H5N1 Vaccine with Lycium barbarum Polysaccharide as an Adjuvant

作　　者：刘为[1] 许馨月[1] 张风华[1] 陈则[1,2] 方芳[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙41008 [2]上海生物制品所,上海200052

出　　处：《激光生物学报》2011年第3期338-341,共4页Acta Laser Biology Sinica

基　　金：国家支撑计划(2006BAD06A03);湖南省科技厅科研计划项目(2006NK2003);湖南省教育厅一般项目(07C564);上海市科委科研计划项目(0643 19030)

摘　　要：探讨枸杞多糖(Lycium barbarum polysaccharide,LBP)作为佐剂对H5亚型流感病毒全病毒灭活疫苗的体液免疫增强效果。将流感病毒A/Vietnam/1194/2004(H5N1)灭活疫苗与不同剂量的枸杞多糖混合后以腹腔注射的方式共同免疫小鼠,免疫后三周收集血清用于特异性抗体检测。实验中设立氢氧化铝佐剂组作对照共同评价LBP作为佐剂的免疫增强效果。结果显示,小鼠血清中针对H5灭活疫苗的特异性抗体水平在一定范围内随着LBP剂量的增加而提高。LBP在800μg剂量时血清特异性抗体水平较无佐剂组显著增强,并与氢氧化铝佐剂组大致相当。因而,LBP有可能成为一种有效的流感灭活疫苗免疫佐剂。Humoral immune effects induced by inactivated whole-virion H5 N1 vaccine adjuvanted with Lycium barbarum polysaccharide （LBP） were explored in BALB/c mice. Mice were intraperitoneally immunized with the whole-virion inactivated influenza virus A/Vietnam/1194/2004 （ H5N1 ） vaccine formulated with different dosages of Lycium barbarum polysaccharide （ LBP）. Three weeks after the immunization, mice were bled for detection of serum vaccine-specific antibody levels, Mice injected with aluminium hydroxide-adjuvanted inactivated vaccine were used as positive control. Results showed that serum specific antibody titers in mice were increased along with the increasing amount of LBP within a certain range. Compared with the non-adjuvanted vaccine, the vaccine adjuvanted with LBP at dosage of 800 p,g significantly enhanced the antibody level. The adjuvant effect of LBP on humoral responses was comparable to that of aluminium hydroxide. LBP may be a potential adjuvant to inactivated influenza virus vaccines.

关 键 词：枸杞多糖 流感 灭活疫苗 佐剂 

分 类 号：R373[医药卫生—病原生物学]