转染GFP对结肠直肠癌细胞遗传物质稳定性的影响  

The Influence of Green Fluorescent Protein(GFP) on Genomic Stability in Colorectal Cancer Cells

作　　者：李艳杰[1,2] 吴珏[1,2] 杨超[1,2] 

机构地区：[1]安徽师范大学安徽省重要生物资源保护与利用重点实验室,安徽芜湖241000 [2]安徽师范大学生物环境与生态安全安徽省高校重点实验室,安徽芜湖241000

出　　处：《激光生物学报》2011年第3期342-346,共5页Acta Laser Biology Sinica

基　　金：安徽省自然科学基金(070413140)

摘　　要：为探究绿色荧光蛋白(GFP)对结肠直肠癌细胞遗传物质稳定性是否存在影响,选取3种常用的结肠直肠癌细胞系HCT116,SW480,DLD-1;采用脂质体转染法把GFP转入细胞,统计几种细胞系微核、核芽的比例;分别比较各种细胞系自发微核、核芽和GFP组的差异。结果发现,HCT116细胞中对照组与GFP组的微核细胞率分别为3.24%、2.76%,核芽细胞率为0.24%、0.26%;SW480细胞中对照组与GFP组的微核细胞率分别为3.49%、3.58%,核芽细胞率为1.48%、1.33%;DLD-1细胞中对照组与GFP组的微核细胞率分别为1.12%、1.28%,核芽细胞率为0.50%、0.56%。三种细胞系中自发微核、核芽数目和GFP组经2×2卡方检验,P值均大于0.05,它们之间没有显著性差异。实验结果表明,转染GFP不影响三种细胞系的遗传物质稳定性,GFP可以作为一种安全可靠的标记物用于哺乳动物细胞核的标记。The objective was to investigate the effect of the green fluorescent protein （GFP） on cell＇s genomic stability. Three well-known colorectal cancer cell lines （ HCT116, SW480, DLD-1 ） were transfected with GFP. We investigated micronucleus and nucleus bud in GFP and control group. The ratio of micronucleus and nucleus bud in HCT116/ HCT116-GFP groups are 3.24 % , 2.76 % and 0.24 % , 0.26 % ; SW480/SW480-GFP groups are 3.49 % , 3.58 % and 1.48 % , 1.33 % ; DLD-1/DLD-1-GFP groups are 1.12 % , 1.28 % and 0.50 % , 0. 56 % ; respectively. The statistical data between control and GFP group have no statistically significance （P 〉 0.05）. Based on these results, we conclude that GFP as a kind of reporter gene have no effect on genonaic stability in three well-known colorectal cancer ceil lines. GFP can be used as a security marker to tag the nucleus of mammal cells.

关 键 词：绿色荧光蛋白 结肠直肠癌细胞 微核 核芽 

分 类 号：Q291[生物学—细胞生物学]