龟纹瓢虫四虫态肠道细菌分离及鉴定  

The isolation and identification of intestinal bacteria of four states of Propylea japonica

作　　者：赵秀芝[1] 刘玉升[1] 张帆[2] 

机构地区：[1]山东农业大学植物保护学院环境生物与昆虫资源研究所,山东泰安271018 [2]北京市农林科学院植保环保所,北京100097

出　　处：《中国微生态学杂志》2011年第6期500-504,共5页Chinese Journal of Microecology

基　　金：国家"973"计划项目(2009CB119206)

摘　　要：目的通过对龟纹瓢虫四个虫态肠道菌群研究,探讨其营养生理,为改良龟纹瓢虫人工饲料,大量扩繁天敌昆虫奠定基础。方法按照传统的分离方法从龟纹瓢虫瓢卵、幼虫、蛹及雌成虫或肠道内分离15个不同菌株,对其染色反应、培养性状,菌体形态和生理生化性状进行系统研究。结果从龟纹瓢虫卵内分离鉴定出短芽胞杆菌(Bacillus brevis)、球性芽胞杆菌(Bacillus sphaericus)、棍状杆菌属(Clavibacter)和一种未知分类地位的菌;幼虫肠道内分离鉴定柠檬酸杆菌属(Citrob acter)、短状杆菌属(Brachybacterium)、微杆菌属(Microbacterium)、皮杆菌属(Dermabacter)、浸麻芽胞杆菌属(Bacillus macerans)、纤维单胞菌属(Cellulomonas);蛹肠道分离鉴定出短芽胞杆菌(Bacillus brevis)、蜡状芽胞杆菌(Bacillus cereus)、微杆菌属(Microbacterium)、不动杆菌属(Acinetobacter);成虫肠道分离鉴定出短芽胞杆菌(Bacillus brevis)、球性芽胞杆菌(Bacillus sphaericus)、柠檬酸杆菌属(Citrob acter)、不动杆菌属(Acinetobacter)、地衣芽胞杆菌(Bacillus licheniformis)、凝结芽胞杆菌(Bacillus coagulans)和巨大芽胞杆菌(Bacillusmegaterium)。结论不同虫态龟纹瓢虫肠道中细菌种类不同,为进一步研究适合龟纹瓢虫不同时期生长和繁殖的人工饲料的配比提供了依据。Objective To intensively study the nutritional physiology of Propylea japonica egg,1arvae,pupae and adult gut and analyze their digestion mechanism,and lay a foundation for further study on the Ratio of artificial feed and breed more natural enemies.Method Fifteen bacterial strains were isolated from the intestine of Propylea japonica egg,1arvae,pupae and adult by traditional methods of separation,purification and cultivation.Their morphology,staining reactions,culture traits,physiological and biochemical reactions were studied systematically.Result Total 4 different isolates of bacteria were obtained from egg intestine.These bacteria belong to Bacillus brevis,Bacillus sphaericus,Clavibacter and an unidentified strain;6 different isolates of bacteria were obtained from1arvae intestine,which belong to Citrob acter,Brachybacterium,Microbacterium,Dermabacter,Cellulomonas and Macerans;4 bacteria strains were isolated from the intestine of pupae,which were identified as Bacillus brevis,Bacillus cereus and Microbacterium acinetobacter.7 different isolates of bacteria were obtained from adult intestine,which belong to Bacillus brevis,Bacillus sphaericus,Citrob acter,Acinetobacter,Bacillus licheniformis,Megaterium and Bacillus coagulans.Conclusion The kinds of intestinal microbial flora in the four states are related with living behavior of Propylea japonica.The results provides a foundation for further research of Propylea japonica＇s artifical diet,development and reproduction.

关 键 词：龟纹瓢虫 四虫态 肠道细菌 鉴定 

分 类 号：R996.496[医药卫生—毒理学]