链霉菌2754发酵产物的分离提取及结构鉴定  

Isolation,purification and structure identification of products from Streptomyces 2754

作　　者：刘祺[1] 蒋忠科[1] 郭连宏[1] 张洋[1] 吴剑波[1] 姜蓉[1] 

机构地区：[1]中国医学科学院&北京协和医学院医药生物技术所,北京100050

出　　处：《中国抗生素杂志》2011年第6期412-415,共4页Chinese Journal of Antibiotics

基　　金：2009年中央级公益性科研院所基本科研业务费专项资金;科技部十一五"重大新药创制"科技重大专项-综合性新药研究开发技术大平台(2009zx09301)资助

摘　　要：目的从链霉菌2754中提取分离和纯化小分子VEGF受体抑制剂,并进行结构鉴定。方法以酪氨酸酶活性测定方法(ELISA法)为活性跟踪方法,利用大孔吸附树脂反相HPLC等方法进行分离纯化。利用光谱分析,进行结构解析。结果分离纯化到两个七尾霉素类化合物nanaomycinαA和nanaomycinαA并具有相关活性。结论 NanaomycinαA和nanaomycinαA为已知化合物,首次报道该化合物具VEGFR拮抗活性。Objective To isolate, purify and determine the structure of inhibitors of VEGFR. Methods We use the ELISA method to find compounds, and Macroporous Resin, RP-PCR to isolate and purify compounds. The structure was determined by spectral analysis. Results Obtained two nanaomycin analogues： nanaomycinαA and nanaomycin βA, both of them have bioactivities. Conclusions NanaomycinαA and nanaomycin βA are known compounds. It is firstly reported about its antagonistic activity of VEGFR.

关 键 词：VEGFR C2754 分离纯化 结构鉴定 

分 类 号：R979.1[医药卫生—药品]