盐酸奎宁与全氟辛烷磺酸体系的共振光散射光谱研究及其分析应用  

Study on the resonance light scattering spectra of the interaction of quinine dihydrochloride with perfluorooctane sulfonate and its analytical applications

作　　者：吴飞[1] 谭克俊[1] 刘忠德[2] 

机构地区：[1]发光与实时分析教育部重点实验室 [2]西南大学化学化工学院,重庆400715 [2]西南大学药学院,重庆400715

出　　处：《中国科学：化学》2011年第7期1163-1169,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(20877063)资助

摘　　要：研究了盐酸奎宁(Quinine dihydrochloride,简称Quinine)与全氟辛烷磺酸(perfluorooctane sulfonate,简称PFOS)相互作用的共振光散射(resonance light scattering,RLS)光谱,并建立了PFOS的共振光散射分析方法.在pH值为2.87的Britton-Robinson(BR)缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的盐酸奎宁通过静电引力和疏水作用形成2:1的离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于283nm处,增强的散射信号强度与PFOS浓度在0.10～50.0μmol/L范围内呈线性关系,据此建立了测定PFOS的散射分析方法,检测限为9.88nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,同时研究了体系的吸收光谱及荧光光谱,并探讨了反应机理.本方法用于水样及人体血清样品中PFOS的测定,RSD≤4.2%.The resonance light scattering（RLS） spectra of the interaction Quinine dihydrochloride （Quinine）with perfluorooctane sulfonate （PFOS） was investigated.A RLS method for the determination of PFOS has been established.In pH 2.87 Britton-Robinson （BR） buffer solution,perfluorooctane sulfonate （PFOS） anions can react with the protonated Quinine by electrostatic forces and hydrophobic interactions to form 2：1 ion-association complexes and resulting in greatly enhanced resonance light scattering signals characterized by a peak at 283 nm,and the RLS intensity was proportional to the concentration of PFOS in the range of 0.10 ～ 5.00 μmol/L.The limit of detection is 9.88 nmol/L.In this paper,the optimum reaction conditions and the interference of foreign substances of the system were investigated.The absorption and fluorescence spectra of the system as well as the reaction mechanism were also discussed.This RLS method has been applied to the determination of PFOS in environmental samples and human serum samples with RSD ≤ 4.2%.

关 键 词：全氟辛烷磺酸 盐酸奎宁 共振光散射 吸收光谱 荧光猝灭 

分 类 号：O621.2[理学—有机化学]