HPLC法测定癌痛巴布剂中乌头碱、次乌头碱、新乌头碱  

Determination of aconitine,mesaconitine and hypaconitine in Aitong Cataplasm by HPLC

作　　者：李智勇[1] 邓亚利[2] 孙冬梅[1] 

机构地区：[1]广东省中医研究所,广东广州510095 [2]华南农业大学制药工程系,广东广州510642

出　　处：《中成药》2011年第6期988-991,共4页Chinese Traditional Patent Medicine

基　　金：广东省中医药局建设中医药强省科研经费资助项目(2008220)

摘　　要：目的建立癌痛巴布剂(草乌、生天南星、赤芍、乳香、生姜,等)中乌头碱、次乌头碱、新乌头碱的定量测定方法。方法色谱柱Elipse CDB-C18(4.6mm×150mm,5μm)键合硅胶柱;流动相乙腈-0.2%冰乙酸(加三乙胺调节pH值为6.25)(29∶71);体积流量1.0 mL/min;柱温30℃;检测波长235 nm。采用HPLC法测定癌痛巴布剂中乌头碱、次乌头碱、新乌头碱。结果乌头碱、次乌头碱、新乌头碱分别在进样量53.6～321.6 ng、145.2～871.2 ng、326.4～1 598.4 ng范围内线性关系良好,r均为1,在8 h内稳定性良好。平均加样回收率分别为99.26%(RSD为1.83%)、99.71%(RSD为1.52%)、97.82%(RSD为1.15%)(n=6)。结论该方法准确、简便、快速,可以用于定量测定癌痛巴布剂中乌头碱、次乌头碱、新乌头碱。AIM To develope an HPLC method for determining aconitine,mesaconitine and hypaconitine in Aitong Cataplasm（Aconiti kusnezoffii Radix,Arisaematis Rhizoma,Paeoniae Radix rubra,Olibanum,Zingiberis Rhizoma recens,etc）.METHODS HPLC was used for the determination of aconitine,mesaconitine and hypaconitine.The column was Elipse CDB-C18（4.6 mm×250 mm,5μm）;the mobile phase was acetonitrile-0.2% acetic acid（29∶71）（adjusted by triethylamine pH value at 6.25）;the detection wavelength was set at 235 nm;the flow rate was 1.0mL/min.RESULTS Aconitine,mesaconitine and hypaconitine showed a good linear relationship in the ranges of 53.6-321.6 ng（r=1）、145.2-871.2 ng（r=1） and 326.4-1 598.4 ng（r=1）,respectively,and their average recoveries were 99.26%,99.71% and 97.82%,also with RSDs of 1.83%,1.52%,1.15%（n=6）,respectively.CONCLUSION This method is accurate,simple,reliable and can be used for the determination of aconitine,mesaconitine and hypaconitine in Aitong Cataplasm.

关 键 词：癌痛巴布剂 HPLC 乌头碱 次乌头碱 新乌头碱 

分 类 号：R927.2[医药卫生—药学]