新型糖基化酶AlkC和AlkD  

Research progress of two novel DNA glycosylases:AlkC and AlkD

作　　者：魏建科[1,2] 关文[2] 朱静[2] 曹春阳[2] 龚福春[1] 

机构地区：[1]长沙理工大学化学与生物工程学院,长沙410114 [2]中国科学院上海有机化学研究所,上海200032

出　　处：《生命的化学》2011年第3期415-418,共4页Chemistry of Life

基　　金：国家自然科学基金项目(20902103;20872169和20921091);上海市浦江计划人才项目(08PJ1411700);科技部重大研究计划项目(2009CB918600)资助

摘　　要：DNA糖基化酶是一类有着重要生物功能的蛋白质,广泛存在于原核生物和真核生物中。研究表明,DNA糖基化酶能够特异性地识别损伤碱基,再通过各种酶修复DNA。最近,科学家在筛选土壤中3-甲基腺嘌呤糖基化酶时,发现了三种基因AlkC、AlkD和AlkE,其中AlkC和AlkD是两种新基因。本文根据近年来的研究成果,对AlkC和AlkD两种碱基糖基化修复酶的结构和功能,以及AlkD的切除损伤DNA的核酸捕获机理进行了总结。Existing in prokaryotes and eukaryotes,DNA glycosylase is a kind of protein which has very important biological functions.They can recognize specific lesion bases in DNA and repair it by hydrolyzing C-N glycosidic bond of the damaged site in DNA.Recently scientists have discovered three genes,AlkC,AlkD and AlkE during screening 3-methyladenine DNA glycosylase in genomic libraries of the soil bacterium.Among them,AlkC and AlkD were discovered for the first time.In this paper,a detailed review on the research progress of DNA glycosylases,especially on the function and the structure of AlkC and AlkD,was made.A novel lesion capture mechanism of AlkD was also discussed.

关 键 词：DNA糖基化酶 AlkC AlkD 

分 类 号：Q55[生物学—生物化学]