大蒜油对正己烷染毒小鼠毒性拮抗作用  

Antagonistic effect of garlic oil on toxicity of n-hexane in mice

作　　者：高绪聪[1,2] 谢克勤[2] 

机构地区：[1]天津药物研究院新药安全评价研究中心,天津300193 [2]山东大学公共卫生学院毒理学研究所

出　　处：《中国公共卫生》2011年第7期883-884,共2页Chinese Journal of Public Health

基　　金：国家重点基础研究发展计划(973计划)项目(2002CB512907)

摘　　要：目的探讨大蒜油减少小鼠正己烷活性代谢产物的生成作用,为减少正己烷中毒提供实验依据。方法健康成年昆明小鼠,随机分为正己烷染毒组和大蒜油干预组,分别灌胃给予正己烷和大蒜油+正己烷,取血,分离血清经乙酸乙酯萃取后,气相色谱法测定血清中2,5-己二酮(2,5-HD)含量。结果小鼠经正己烷单次灌胃染毒后测定血清2,5-HD含量为(0.14±0.14)μg/mL,10 h后达峰值(24.63±3.52)μg/mL,至染毒后20 h降至(0.02±0.04)μg/mL;正己烷染毒前后给予大蒜油使小鼠6h血清2,5-HD含量分别较单纯染毒组降低32.1%(P<0.01)、24.4%(P<0.05);提前给予大蒜油使小鼠4～14 h含量降低26%～54%不等,其中染毒后4、6、8、10 h与单纯染毒组比较,差异有统计学意义(P<0.05或P<0.01),但大蒜油并未改变正己烷染毒小鼠2,5-HD含量曲线的形状和达峰时间。结论大蒜油可抑制正己烷染毒小鼠2,5-HD的产生,降低血清2,5-HD水平,降低正己烷毒性。Objective To investigate antagonoistic effect of garlic oil（GO） on toxicity of n-hexane by decreasing the formation of 2,5-hexanedione（2,5-HD） in mice.Methods Adult healthy Kunming mice were treated with n-hexane and GO＋ n-hexane,respectively.The serum was obtained and extracted with ethyl acetate,and the levels of the serum 2,5-HD were determined with gas chromatography.Results The content of 2,5-HD in serum of the mice detected immediately after a single exposure of n-hexane was 0.14±0.14 μg/ml with the peak values of 24.63±3.52 μg/ml at 10 hr and 0.02±0.04 μg/ml at 20 hr.The serum 2,5-HD decreased by 32.1%（P0.01） and 24.4%（P0.05） at 6 hr after GO pre-treatment and post-treatment.Compared to that of the n-hexane group,the content of serum 2,5-HD in GO pretreated group decreased by 26%-54% within 4 hr-14 hr after the administration of n-hexane,with significant differences at 4,6,8 and 10 hours（P0.05 or P0.01）,but the shape and peak time of the curve were not changed.Conclusion GO can significantly inhibit the metablism of n-hexane with the decrease of serum 2,5-HD and result in the deacrease of the toxicity of n-hexane.

关 键 词：大蒜油 正己烷 2 5-己二酮 

分 类 号：R994.3[医药卫生—毒理学]