苯并芘对大鼠记忆及谷氨酸盐受体基因表达影响  

Effect of benzo(a) pyrene on memory and expression of glutamate receptor mRNA in rat

作　　者：唐倩[1] 夏茵茵[1] 程淑群[1] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生学教研室,重庆400016

出　　处：《中国公共卫生》2011年第7期902-903,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671744)

摘　　要：目的观察慢性苯并芘染毒大鼠对记忆及海马组织中谷氨酸盐受体基因(AMPA1)表达影响。方法将36只雄性SD大鼠分为高、中、低剂量组及溶剂对照组,每组9只;染毒结束后,用Morris水迷宫测试大鼠空间学习记忆能力,基因芯片技术进行AMPA1基因表达谱分析,再用逆转录-实时荧光定量PCR技术测量大鼠海马组织中AMPA1基因表达状况。结果第5 d定位航行试验中,与对照组(6.50±2.57)s比较,中、高剂量组寻找平台平均潜伏期(17.90±7.71)、(16.20±4.74)s,明显延长(P<0.05);基因芯片扫描提示AMPA1 mRNA在暴露组中表达量是对照组的2.052倍,荧光定量PCR检测显示AMPA1mRNA上调4.257倍。结论苯并芘影响大鼠的空间学习记忆能力,且明显诱导海马组织内的AMPA1基因高表达。Objective To investigate the effect of chronic administration of benzo（a）pyrene on memory and the expression of glutamate receptor（2-amino-3-hydroxy-5-methyl-4-isoxazol propionate subunit 1,AMPA1）mRNA in hippocampus of rats.Methods Thirty-six healthy male rats were randomly divided into high,moderate and low dose toxin exposure groups and vehicle group（9 in each group）.Afterwards,the rats were tested for learning and memory（Morris water maze）,and the high exposure and vehicle group were studied with gene microarray screening and real-time PCR for AMPA1 mRNA in hippocampus.Results Compared with the control group（6.50±2.57s）,latency in finding the platform of the rats in high exposure group（17.90±7.71s） and moderate exposure group（16.20±4.74s） increased significantly（P0.05） on the fifth day of place navigation.The cDNA microarray analysis showed that AMPA1 mRNA level in high exposure group was 2.052 times higher than that of the vehicle group.Real-time PCR showed that AMPA1 mRNA was up-regulated by 4.257 times.Conclusion Benzo（a）pyrene had an inhibitive effect on spatial learning and memory capability,and significantly increase gene expression of AMPA1 subunit in hippocampus of rats.

关 键 词：苯并芘 大鼠 海马组织 谷氨酸盐受体基因 

分 类 号：R996[医药卫生—毒理学]