Survivin和bax在人乳腺癌MCF-7细胞中表达及中药干预作用  

Effect of total glycosides of Solamum lyratum on expression of survivin and bax in MCF-7 human breast cancer cells

作　　者：杨旭东[1] 张杰[2] 赵容杰[1] 

机构地区：[1]牡丹江医学院药理教研室,牡丹江157011 [2]牡丹江医学院生化教研室,牡丹江157011

出　　处：《中国新药杂志》2011年第12期1123-1126,共4页Chinese Journal of New Drugs

基　　金：黑龙江省教育厅科学技术研究项目(11531398)

摘　　要：目的:探讨survivin和bax基因在白毛藤总苷诱导人乳腺癌系MCF-7细胞凋亡中的作用。方法:不同浓度白毛藤总苷作用于MCF-7细胞48 h后,荧光显微镜和流式细胞术法检测MCF-7细胞凋亡情况,Western印迹法及RT-PCR法检测survivin和bax基因表达量的变化。结果:白毛藤总苷能诱导MCF-7细胞凋亡,并且上调bax和降低survivin表达。结论:白毛藤总苷能促进MCF-7细胞凋亡,其机制可能与激活bax和抑制survivin表达有关。Objective： To investigate the effects of total glycosides of Solamum lyratum on expression bax and survivin in the human breast cancer line,MCF-7 cells.Methods： MCF-7 cells were treated with total glycosides of S.lyratum at various concentrations.Apoptosis of MCF-7 cells was observed by fluorescence microscope and flow cytometry（FCM）,and the expression levels of bax and survivin were detected by Western blotting and RT-PCR method.Results： The total glycosides of S.lyratum obviously induced apoptosis of MCF-7 cells,increased bax expression,and decreased the level of survivin expression.Conclusion： The total glycosides of S.lyratum can induce apoptosis of MCF-7 cells probably through down-regulating bax and up-regulating survivin expression.

关 键 词：白毛藤总苷 乳腺癌 细胞凋亡 

分 类 号：R286.91[医药卫生—中药学]