应用离体牛蛙肺泡膜模型研究胰岛素的肺吸收特性  

Application of Rana catesbeiana pulmonary membrane in the study of insulin pulmonary absorption characteristics

作　　者：方芳[1] 杨硕晔[1] 郑剑恒[1] 何佳珂[1] 李宁[1] 陈西敬[1] 汤玥[2] 朱家壁[2] 

机构地区：[1]中国药科大学药物代谢动力学研究中心,南京210009 [2]中国药科大学药物制剂研究所,南京210009

出　　处：《中国新药杂志》2011年第12期1127-1130,1135,共5页Chinese Journal of New Drugs

基　　金：国家高技术研究发展计划(863计划)(2007AA02Z171)

摘　　要：目的:建立离体牛蛙肺泡膜模型并初步探讨胰岛素的肺吸收机制。方法:建立离体牛蛙肺泡膜模型,对模型的完整性、通透性及影响因素等进行考察,并初步研究胰岛素的肺吸收机制。结果:在4 h内,膜的紧密性和通透性良好,在一定范围内胰岛素透过牛蛙肺泡膜是非浓度、非方向依赖性的,且单体较六聚体透过率高。结论:离体牛蛙肺泡膜模型可作为评价胰岛素肺吸收的体外模型。经比较,单体胰岛素可能比六聚体胰岛素肺吸收好。Objective： To establish a Rana catesbeiana alveolar membrane model and to investigate the pulmonary absorption characteristics of insulin using this model.Methods： In the model of Rana catesbeiana alveolar membrane,the integrity,permeability and influence factors were investigated.The mechanism of insulin pulmonary absorption was studied.Results： During 4 h,the integrity and permeability of Rana catesbeiana pulmonary monolayer were satisfactory.Insulin permeated the alveolar membrane in a concentration-and direction-independent manner,and monomeric insulin was easier to be absorbed into the lung than hexameric insulin.Conclusion： Insulin can passively diffuse through the barrier of Rana catesbeiana alveolar membrane via a paracellular pathway.Rana catesbeiana alveolar membrane,as a transport model,has the potential to investigate the pulmonary absorption mechanism of insulin.

关 键 词：胰岛素 牛蛙肺泡膜 肺吸收 转运 

分 类 号：R977.15[医药卫生—药品] R965[医药卫生—药学]