金属硫蛋白与心肌血管内皮细胞的特异结合  

SPECIFIC BINDING SITES OF METALLOTHIONEIN ON ENDOTHELIAL CELLS OF SMALL VESSELS IN MYOCARDIUM

作　　者：葛淑君[1] 仲崇霞[1] 程时[1] 

机构地区：[1]北京医科大学生物物理系

出　　处：《解剖学报》1999年第3期248-250,I012,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：目的探讨金属硫蛋白（ＭＴ）进入细胞的机制。方法以胶体金为示踪物，采用离体心脏灌流术，电镜下观察心肌血管内皮细胞对ＭＴ的摄入。结果心肌血管内皮细胞能特异地摄取ＭＴ。结论血管内皮细胞表面存在ＭＴ特异结合位点，为深入研究该蛋白的生理功能及其应用提供了重要依据。Objective\ To explore the mechanism of metallothionein (MT) entering cells.Methods\ Using colloidal gold labelling and isolated heart perfusion technique,uptaking process of MT by endothelial cells of small vessels in myocardium was observed by electron microscopy.Results\ Endothelial cells of the vessels in myocardium are able to specially uptake colloidal gold particles.Conclusion\ There might be specific binding sites of MT on endothelial cell surface of small vessels in myocardium,which is very important for revealing MT biological function and clinical use.

关 键 词：金属硫蛋白 内皮细胞 特异结合位点 心肌血管 

分 类 号：Q513[生物学—生物化学]