实验性脾虚证大鼠胰岛细胞的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY ON THE PANCREATIC ISLET CELLS IN EXPERIMENTAL SPLEEN DEFICIENCY SYNDROME OF RATS

作　　者：王秀琴[1] 尚宏伟[1] 曾晓蓓[1] 孟庆峰[1] 王兴翠[1] 杨进[1] 

机构地区：[1]首都医科大学组织学胚胎学教研室

出　　处：《解剖学报》1999年第3期251-255,I013,共6页Acta Anatomica Sinica

基　　金：北京市中医管理局资助

摘　　要：目的研究实验性脾虚证大鼠胰岛细胞胰岛素、胰岛淀粉样多肽、胰高血糖素和生长抑素分泌活动的变化，探讨中医学脾虚证发病与胰岛激素分泌活动的关系。方法用Ｗｉｓｔａｒ雄性成年大鼠２４只，分为正常对照组、实验性脾虚组、自然恢复组和中药治疗组。４组动物同时麻醉处死，取胰尾固定于Ｂｏｕｉｎ液，制成石蜡切片。用免疫组织化学法显示胰岛Ｂ细胞胰岛素和胰岛淀粉样多肽、Ａ细胞胰高血糖素和Ｄ细胞生长抑素，并用显微分光光度计对免疫染色强度进行了定量测定。结果与对照组相比，脾虚组胰岛Ｂ细胞胰岛素和Ａ细胞胰高血糖素免疫染色明显减弱，而Ｄ细胞生长抑素和Ｂ细胞胰岛淀粉样多肽免疫染色明显增强。结论上述结果提示，胰岛细胞激素分泌活动失常可能与脾虚证代谢异常有密切关系。Objective\ To study the relationship between the islet hormonal secretory activity and the pathogenesis of spleen deficiency syndrome in Chinese traditional medicine.Methods\ The changes of secretory activity of insulin(Ins), islet amyloid polypeptide (IAPP),glucagon(Glu) and somatostatin (SOM) in pancreatic islet cells of experimental spleen dificiency syndrome rats were observed by immunohistochemical technique.24 adult male Wistar rats were divided into 4 groups:(1)normal control group; (2)experimental spleen deficiency syndrome group induced by rhubarb combined with imbalance of food supply;(3) therapeutic group treated with Chinese herbs “Plus Si Jun Zi Decoction”,and (4) self recovery group,that is,the syndrom was induced but without medical treatment.The tail portions of pancreas were taken and prepared for immunohistochemical demonstration of A cells (for Glu),B cells(for Ins and IAPP) and D cells (for SOM).The immunoreactivity of the above mentioned cells were measured by microspectrophotometry.Results\ As compared with the normal control,the immunereactivity of Ins in B cells and Glu in A cells of the experimental spleen deficiency syndrome group is obviously declined, while IAPP in B cells and SOM in D cells are apparently elevated.Conclusion\ This study suggests that the disorder of islet cell secretory activity seems to be intimately related to the metabolic abnormality in the spleen deficiency syndrome.

关 键 词：胰岛细胞 实验性 脾虚证 免疫组织化学 大鼠 

分 类 号：R256.302[医药卫生—中医内科学]