山绿茶药材质量标准的研究  

Study on quality standard of Ilex hainanensis Merr.

作　　者：陈勇[1] 谢臻[1] 李立 李兵[1] 李耀华[1] 韦韬[1] 

机构地区：[1]广西中医学院药学院,南宁530001 [2]广西南宁食品药品检验所,南宁530001

出　　处：《中华中医药杂志》2011年第7期1606-1608,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西科学研究与技术开发计划项目(No.桂科攻032202424);广西自然科学基金项目(No.桂科自054297)~~

摘　　要：目的:建立山绿茶药材的质量标准。方法:采用TLC法鉴别山绿茶药材中芦丁;采用HPLC法测定山绿茶药材中绿原酸与芦丁的含量。结果:TLC鉴别芦丁斑点清晰,绿原酸进样量在0.01082-0.8656μg之间与峰面积呈良好的线性关系,r=1.000,平均回收率为96.53%,RSD为1.41%。芦丁进样量在0.02028-1.6224μg之间与峰面积呈良好的线性关系,r=0.9997,平均回收率为99.15%,RSD为2.00%。结论:所建立的质量控制方法灵敏,简便,重现性好,可用于山绿茶药材的质量控制。Objective： To establish the method for quality control of Ilex hainanensis Merr..Methods： Rutin of Ilex hainanensis Merr.was identified by TLC.HPLC was used to determine the contents of rutin and chlorogenic acid.Results： Rutin spot on TLC plates was clear.The linear range of chlorogenic acid was good from 0.01082μg-0.8656μg,r=1.000,the average recovery was 96.53%（RSD=1.41%）.The linear range of rutin was good from 0.02028μg-1.6224μg,r=0.9997,the average recovery was 99.15%（RSD=2.00%）.Conclusion： The method was accurate,simple and reproducible which can be applied to the quality control of Ilex hainanensis Merr..

关 键 词：山绿茶 薄层色谱 高效液相色谱 芦丁 绿原酸 

分 类 号：R282.71[医药卫生—中药学]